Regular ArticleCharacterization of Variability in Large-Scale Gene Expression Data: Implications for Study Design
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The supplementary data, HudsonGENO2001-0291Sup.doc, is a Microsoft Word document, which contains Appendix A: Description of Analytic Methods.
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2016, GeneCitation Excerpt :We can thus roughly categorize HK genes into two subclasses: constantly expressed HK genes and variably expressed HK genes. In this study, a coefficient variation (CV) model was used to assess the expression variation for each gene in distinct tissues (De Jonge et al., 2007; Novak et al., 2002), and the calculation results showed that CV scores of expression level for all genes ranged from 0.15 to 3.79 (Fig. 3A). According to the distribution, we adopted the following two criteria to define constant HK genes: (1) less than a quarter of a quantile of CV scores (0.47); (2) the maximum fold change of expression levels across tissues < 2 (log2 (FPKMy/FPKMx) < 1) (Eisenberg and Levanon, 2013; De Jonge et al., 2007).
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