Use of Flow-Sorted Canine Chromosomes in the Assignment of Canine Linkage, Radiation Hybrid, and Syntenic Groups to Chromosomes: Refinement and Verification of the Comparative Chromosome Map for Dog and Human

doi:10.1006/geno.2000.6334

Genomics

Volume 69, Issue 2, 15 October 2000, Pages 182-195

https://doi.org/10.1006/geno.2000.6334 Get rights and content

Abstract

The mapping of the canine genome has recently been accelerated by the availability of chromosome-specific reagents and publication of radiation hybrid (RH), genetic linkage, and dog/human comparative maps, but the assignment of mapping groups to chromosomes is incomplete. To assign published radiation hybrid, linkage, and “syntenic” groups to chromosomes, individual markers found within each group have been amplified from canine and vulpine flow-sorted, chromosome-specific DNAs as templates. Here a further 102 type I genetic markers (previously mapped in human) and 21 further type II markers are assigned to canine chromosomes using marker-specific PCR. We have assigned all linkage, RH, and syntenic groups in the two most recently published canine genome maps to chromosomes. This demonstrates directly that there is at least one published mapping group for each of the 38 canine autosomes and thus that the coverage of the canine chromosome map is approaching completion. The dog/human comparative map is one of the most complex so far described, with 90 separate segments of chromosomal homology previously seen in dog-on-human cross-species chromosome-painting studies. The total of 142 type I markers now placed on canine chromosomes using this method of marker mapping has allowed us to confirm the placement of the great majority (83) of the 90 homologous segments. The positions of the remaining homologous segments were confirmed in new cross-species chromosome-painting experiments (dog-on-human, fox-on-human).

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¹: To whom correspondence should be addressed at the Centre for Veterinary Science, Department of Clinical Veterinary Medicine, University of Cambridge, Madingley Road, Cambridge CB3 OES, England. Telephone: (+44) 1223 337686. Fax: (+44) 1223 337610. E-mail: [email protected].

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Regular ArticleUse of Flow-Sorted Canine Chromosomes in the Assignment of Canine Linkage, Radiation Hybrid, and Syntenic Groups to Chromosomes: Refinement and Verification of the Comparative Chromosome Map for Dog and Human

Abstract

Genomics

FEBS Lett.

Genomics

Genomics

Immun. Today

Genomics

Genomics

The DAPI banded karyotype of the domestic dog (Canis familiaris) generated using chromosome-specific paint probes

Chromosome Res.

Structure and expression of a divergent canine class I gene

J. Immunol.

Cloning and sequence analysis of cDNA for the canine neurotensin/neuromedin N precursor

Proc. Natl. Acad. Sci. USA

Genetic analysis by chromosome sorting and painting: Phylogenetic and diagnostic applications

Eur. J. Hum. Genet.

A BstUI PCR/RFLP marker in an intron of the canine fibrillin 1 gene

Anim. Genet.

A comparative chromosome map of the Arctic fox, red fox and dog defined by chromosome painting and G-banding

Chromosome Res.

Chromosomal assignment of seven genes on canine chromosomes by fluorescence in situ hybridization

Mamm. Genome

Chromosome specific paints from a high resolution flow-karyotype of the dog

Chromosome Res.

Localization of the CAMKG gene encoding gamma isoforms of multifunctional calcium/calmodulin-dependent protein kinase (CaM kinase) to human chromosome 10 band q22 and mouse chromosome 14

Cytogenet. Cell Genet.

Regular Article
Use of Flow-Sorted Canine Chromosomes in the Assignment of Canine Linkage, Radiation Hybrid, and Syntenic Groups to Chromosomes: Refinement and Verification of the Comparative Chromosome Map for Dog and Human