Elsevier

Genomics

Volume 54, Issue 1, 15 November 1998, Pages 155-158
Genomics

Short Communication
Identification and Chromosomal Assignment of USP1, a Novel Gene Encoding a Human Ubiquitin-Specific Protease

https://doi.org/10.1006/geno.1998.5554Get rights and content

Abstract

We have cloned a novel gene encoding a human ubiquitin-specific protease (USP1). The product, which consists of 785 amino acids with a deduced molecular mass of 88.2 kDa, possesses His and Cys domains that are highly conserved in all members of the ubiquitin-specific processing (UBP) family of proteases. Recombinant USP1 protein showed genuine UBP activity, correctly cleaving Ub-β-galactosidase to produce ubiquitin and β-galactosidase. Chromosomal mapping by fluorescencein situhybridization and radiation hybrid analyses localized the USP1 gene to the p31.3–p32.1 band of chromosome 1. As losses of heterozygosity or amplifications have been observed in the distal region of the short arm of chromosome 1 in some neuroblastomas, breast cancers, and pancreatic adenocarcinomas, the USP1 gene may be a candidate for either the tumor-suppressive or the oncogenic activities associated with that chromosomal region.

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The nucleotide sequence data reported in this paper have been deposited with the DDBJ/EMBL/GenBank Data Libraries under Accession No. AB014458.

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