Elsevier

Genomics

Volume 48, Issue 2, 1 March 1998, Pages 232-241
Genomics

Regular Article
A High-Resolution Metric HAPPY Map of Human Chromosome 14

https://doi.org/10.1006/geno.1997.5140Get rights and content

Abstract

We have mapped 1001 novel sequence-tagged sites on human chromosome 14. The mean spacing between markers is ∼90 kb, most markers are mapped with a resolution of better than 100 kb, and physical distances are determined. The map was produced using HAPPY mapping, a simple and widely applicablein vitroapproach that is analogous to linkage or to radiation hybrid mapping, but that circumvents many of the difficulties and potential artifacts associated with these methods. We show also that the map serves as a robust scaffold for building physical maps using large-insert clones.

References (31)

  • W. Bautsch et al.

    Long-range restriction mapping of genomic DNA

  • P.H. Dear

    HAPPY mapping

  • P.H. Dear et al.

    Happy mapping: A proposal for linkage mapping the human genome

    Nucleic Acids Res.

    (1989)
  • P.H. Dear et al.

    Happy mapping: Linkage mapping using a physical analogue of meiosis

    Nucleic Acids Res.

    (1993)
  • S. Dear et al.

    A sequence assembly and editing program for efficient management of large projects

    Nucleic Acids Res.

    (1991)
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    H. G. GriffinA. M. Griffin

    1

    To whom correspondence should be addressed. Telephone: [44] (1223) 402190. Fax: [44] (1223) 412178. E-mail:[email protected]. ac.uk.

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