Biochemical and Biophysical Research Communications
Regular ArticleHammerhead Ribozymes Targeted to the FBN1 mRNA Can Discriminate a Single Base Mismatch between Ribozyme and Target☆
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Disruption of dengue virus transmission by mosquitoes
2015, Current Opinion in Insect ScienceCitation Excerpt :Trans-cleaving ribozymes have endoribonuclease activity in the presence of divalent cations like Mg2+ and can also perform multiple turn-over kinetics enhancing their catalytic activity [60–62]. Several features make hRz attractive as synthetic anti-arboviral effectors: firstly, hRz are derived from plant viroids, therefore arbovirus vectors do not exhibit any natural resistance against hRz; secondly, the antiviral effect of hRz is mediated through the catalytic activity of the molecule itself and does not require additional host factors [63]; thirdly, typical hRz target sites are 18–19 nucleotides in length, allowing a relatively high target specificity, fourthly, hRz activity is not restricted to a particular cellular compartment; RNA in both the nucleus and the cytoplasm is efficiently attacked, fifthly, hRz exhibit a high degree of target specificity, as single nucleotide polymorphisms can diminish hRz target recognition and cleavage [64]. In mammalian cells, hRz have demonstrated significant replication inhibition of many medically important viruses including human immunodeficiency virus (HIV), DENV2, influenza A virus, and hepatitis C virus (HCV) [65,66••,67,68].
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Chadwick, D. J.Cardew, G.
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Corresponding author: Dr. Michael W. Kilpatrick, Department of Pediatrics, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06030. Fax: (860) 679 1355. E-mail:[email protected].