Table 2

Identification of 15 differentially expressed proteins in the plasma of patients with Fabry disease during enzyme replacement therapy

Protein nameGeneSpot no.Fold changeAccession no.MALDI-TOF-MSMALDI-TOF-MS/MS
NCBI no.*Swiss-Prot.MassMASCOT score†MassMASCOT score‡
Inter-α-trypsin inhibitor heavy chain H4 isoform one precursor ITIH4 187–2.4gi|31542984Q146241 03 5211061 03 53635
Complement C4 C4A 233–3.1gi|40737468P0C0L458 9514658 93247
C4B 366–2.7gi|1314244P0C0L51 89 599881 89 599220
Keratin, type II cytoskeletal 1 KRT1 234–2.1gi|11935049P0426466 1987166 198189
317–3.0gi|1193504966 1986566 19852
319–2.8gi|1193504966 19810166 19884
370–3.7gi|1193504966 1988866 198195
553–5.2gi|1193504966 1985766 19837
637–4.5gi|1193504966 1986566 198157
655–4.1gi|1193504966 19816166 198198
807–11.1gi|1193504966 1986466 19887
Complement C3 C3 343–2.7gi|115298678P010241 88 5691801 88 569202
90 kDa heat shock protein HSP90AB1
HSP90AA1
464–3.2gi|3 06 891P08238
P07900
83 5847983 58479
Carboxypeptidase N catalytic chain precursor CPN1 594–2.2gi|4503011P1516952 5386352 53830
α-enolase ENO1 625–2.2gi|4503571P0673347 4817647 48160
Actin-β ACTB 654–10.3gi|14250401P6070941 3218541 321124
963–7.0gi|1425040141 32114141 321250
Complement C1q subcomponent subunit C C1QC 822–5.5gi|56786155P0274725 9856425 98576
Profilin-1 PFN1 879–5.7gi|4826898P0773715 2168715 21677
Serum amyloid A-1 protein SAA1 897–2.9gi|2 25 986P0DJI811 6754711 67573
Vinculin VCL 204–2.0gi|24657579P182061 17 2341161 17 23436
Fibrinogen, α chain FGA 409–3.4gi|13591823P0267170 6669570 66677
Protein artemis DCLRE1C 237–2.0gi|76496497Q96SD179 41376
Peroxiredoxin-6 PRDX6 810–2.1gi|4758638P3004125 1336525 13340
  • *Proteins were annotated based on data from protein databases (MS, MS/MS-NCBI no. 100326, 10,635,453 sequences and 3,627,481,469 residues; 101112, 12,256,246 sequences and 4,188,691,752 residues).

  • †The protein score is presented as −10*Log(P), where P is the probability of the observed match being a random event. For the mass spectrometry (MS) data, protein scores >64 were considered to be statistically significant (p<0.05).

  • ‡For the matrix-assisted laser desorption/ionisation-time of flight tandem mass spectrometry (MALDI-TOF-MS/MS) data, individual ion scores >33 indicate identity or extensive homology (p<0.05).