Spred 2 | 1 | 1a | aggaagctttttgcactcca | gatagggtttgggggaagg | 65 | Failsafe D |
| 1b | cgtccccttcccaaatct | acgccaagttcacccaataa | 61 | Failsafe D |
2 | | tgccctgtgcttcttcttct | cccacgagttgtatgtctgc | 61 | 10× PCR buffer |
3 | | ttggtagttgccaccagtga | tactgacctggtcccaaacc | 61 | 10× PCR buffer |
4 | | tgcaaaaagaatggaatgtgg | acagcaacagcaaacactgc | 64 | 10× PCR buffer |
5 | | ttttgggaagagcagcaaat | taaaggcaggagcaggagag | 61 | 10× PCR buffer |
6 | 6a | ggagaaggtggggtgttttt | ctttggggtcctcgccta | 61 | 10× PCR buffer |
| 6b | gaggtccccaagcatgacta | aaggcaataagagccatcca | 61 | Failsafe D |
| 6c | ggaccccgagggagactat | aagagacgagttcccctgtg | 61 | 10× PCR buffer |
Spred 3 | 1 | | tgtgttgtccctgtccttcc | acctgacctctgctccacat | 61 | Failsafe D |
2+3 | Exon 2+3 together | atggacgagacgtcacagg | gcctgctacacagtaggtgct | 68 | Failsafe E |
4 | | gattctgagaagcgggaaga | acgtgggactggaggagatt | 61 | 10× PCR buffer |
1 | | ccctcctcctaccacagaga | gaaaagcccattttcaagca | 60 | 10× PCR buffer |
Spry 1 | 2 | 2a | ccccagcatcattgtaatcc | cgaggagcaggtcttttcac | 60 | 10× PCR buffer |
| 2b | tgctattttgtccttagaccaga | ggaacccttcaagtcatcca | 60 | 10× PCR buffer |
| 2c | ccagtccctggtcataggtc | ctaagcacatgcaggttcca | 60 | 10× PCR buffer |
| 2d | accatcctgtttggcctgta | ggtgggaaacagggaaaaat | 60 | 10× PCR buffer |
1 | | acttggcgatctggctttag | caggaggggaagagccaaac | 60 | 10× PCR buffer |
Spry 2 | 2 | 2.1 | attcaggtgggtggagagtg | agctgaccgtgcttatggat | 60 | 10× PCR buffer |
| 2.2 | ctccactcagcacaaacacg | tggctggaaggtaacaccat | 60 | 10× PCR buffer |
| 2.3 | gacaacccatgttcttgcag | gcctaggagtgtctgtgttgc | 60 | 10× PCR buffer |
| 2.4 | cggtgaaatgctttttgtcc | ttttgccttacagaaagccttt | 60 | 10× PCR buffer |
| 2.5 | tttgcaactgtgaaaaacaagt | gataaaagaagacaaaacagctcagt | 60 | Fail safe C buffer |
1 | 1a | atgtgccctgaaatccatgt | actgcgggggagagaagtag | 60 | 10× PCR buffer |
Spry 3 | | 1b | tgtaaacaggccctctccag | tcagcttctcccttcagagc | 60 | 10× PCR buffer |
| 1c | cttcaggccaatccatcatc | catcatcagtggagcagtgg | 60 | 10× PCR buffer |
| 1d | gctgagagcctcctcgatta | ggggactcgaaggagaaaag | 60 | 10× PCR buffer |
1 | | tgtgcccacccattcatag | tgagggaaggaacactgagg | 60 | 10× PCR buffer |
Spry 4 | 2 | | taagtcaaggggagggtgtg | caggatatcaaaatccccagaa | 60 | 10× PCR buffer |
3 | 3a | ggctcccaaggacctgat | ggtgacatcctggtcacagc | 60 | 10× PCR buffer |
| 3b | tagcctggccctgaccac | agcacaggcactcctggtt | 60 | 10× PCR buffer |
| 3c | aaatgcaaggagtgtgcatc | caggcaggtagcagagcag | 60 | 10× PCR buffer |
| 3d | ggtgctctctccgtggtg | ttcttagatgtcagaagagaacca | 60 | 10× PCR buffer |