Table 2

Chromosomal position and sequence information for 42 probes in the NSD1 MLPA kit

Probe	Chr	kb to next probe	Length (nt)	LIG sequence	Clone sequence
Chr, chromosome.
FGFR4 probe	5q35	8	373	CTTGGGTCCCTGAGAGCTGTGAGAAGG	AGATGCGGCTGCTGCTGGCCCTGTTGGGGGT
FGFR4 probe	5q35	35	355	CCTGCTCCTCCAGCGATTCTGTCT	TCAGCCACGACCCCCTGCCATTGGGATCCAGCTCCTTCCC
NSD1 1 Kb 5′ of ex1	5q35	1.4	337	GCAGGAAACAAAATGGAGGCAGCA	GCTCGGGTCTGCCTCCCTGAGCCAAGCCTGT
NSD1 exon 1	5q35	0.9	166	GTCGGCAGCAGCCATGTTTTTCGA	GCTGTAGCAGCTGCTGCTACCCTGACTGGGCTTCGCT
NSD1 exon 2	5q35	56.6	319	CTGCGGAGACTACAGGATTTGGCCTCCATG	ATCAATGTAGAGTATTTAAATGGGTCTGCTGATGGATCAGAAT
NSD1 exon 3	5q35	12.2	301	GTCTACGCCACTGAAGTATGAAGTTGGAGA	TCTCATCTGGGCAAAATTCAAGAGACGCCCATGGTGG
NSD1 exon 4	5q35	6.9	142	GTGGAGGCTTTTGGAGATCCTTCTGA	GAGAGCCTGGGTGGCTGGAAAAGCAATCGTCATGTTTG
NSD1 exon 5	5q35	24.8	283	GTCAGTGACTCTGGAACATCAAAGCCA	TCAAAACCATTACTTTTCTCTTCTGCTTCTAGTCAGAATCACA
NSD1 exon 6	5q35	2.4	265	GGTCAGAGAAGAAACGCCTTAGGAAGCCAA	GCAAGTGGCTTTTGGAATATACAGAAGAATATGATCAGATATT
NSD1 exon 7	5q35	1.5	184	CTTAGGTAAGTTCCCGCTGTGAAGAGGAAA	GCCTTCTAGCCCGAGGTCGATCTAGTGCTCAGAACAAGCAG
NSD1 exon 8	5q35	4.4	238	CGTCAAAGAAAACCAACTAAGAAACTTCTTGA	ATCCAATGATTTAGACCCTGGATTTATGCCCAAGAAGGGGG
NSD1 exon 9	5q35	2.5	292	GGTGTTGTTTTCACTTATTTATAGTGCTATGAAGCT	GGTCACCTGGAGAATGGCATAACTGAATCTTGTGCCA
NSD1 exon 10	5q35	1.5	202	CAAGATATTTGACAAGCCAAGGAAGCG	AAAACGACAGAGGCATGCTGCAGCCAAGATGCAGTGT
NSD1 exon 11	5q35	3.6	247	CGGCCACAAGCCCCAAGGAG	ACTGTTGAGGAAGGTGTAGAACACGATCCCGGGATGCCTGC
NSD1 exon 12	5q35	5.3	445	GTGAAAAATTGGGTGAGCTGCTGTTATGTG	AGGCTCAGTGCTGTGGGGCTTTCCACCTGGAGTGCCT
NSD1 exon 13	5q35	3.0	148	GTGTGTCCAGAAGTACCCACCCACTG	TTATGCAGAACAAGGGCTTCCGGTGCTCCCTCCACATCTGT
NSD1 exon 14	5q35	7.6	229	GTCCTGTGGCATACCACGCCAAT	GACTTTTGCCTGGCTGCTGGGTCAAAGATCCTTGCATCTAA
NSD1 exon 15	5q35	2.1	346	GGAAACTGGTATTGCAATGACTGTAAAGCA	GGCAAAAAGCCACACTACAGGGAGATTGTCTGGGTAAAAG
NSD1 exon 16	5q35	4.0	160	GAGGGTGACGTGAGCAGCAAGGA	TAAGATGGGCAAAGGAGTGGATGGGACATATAAAAAAGGTAACTTTATCC
NSD1 exon 17	5q35	7.0	211	GGCCCAAAAAGAGCTAAGACAGCTGCA	GGAAGACCGAAAGAATGACAAGAAGCCACCACCTTATAAA
NSD1 exon 18	5q35	1.7	400	CGCTGTCAAAACCAGTGCTTTTCCAA	GCGCCAATATCCAGAGGTTGAAATTTTCCGCACATTACAGC
NSD1 exon 19	5q35	1.3	427	GTCTAGGGTGAGCATATAGAAATTAGTGATATCATG	TTCTTGAGCATAGCGAATTCGAGCTCTGCATTCTTCTTCATCTATAAGC
NSD1 exon 20	5q35	5.0	418	GACCGAATCATTGATGCTGGTCCCAA	AGGAAACTATGCTCGGTTCATGAATCATTGCTGCCAGCCCA
NSD1 exon 21	5q35	3.2	454	CTAGAATGTCTTGGGAATGGAAAGACTGTT	TGCAAATGTGGAGCCCCGAACTGCAGTGGCTTCTTGG
NSD1 exon 22	5q35	2.7	364	GGAGCGAGAAGATGAGTGTTTTAGTTGTG	GGGATGCTGGCCAGCTCGTCTCCTGCAAGAAACCA
NSD1 exon 23	5q35	3500	178	CCAGAAGGAGCGGGCAGCTTCACCTCA	TCAGGTCACACCACAGGCTGATGAGAAGATGCCAGTGTTG
FLT4 probe	5q35	629	391	CCTTGAACATCACGGAGGAGTCACACG	TCATCGACACCGGTGACAGCCTGTCCATCTCCTG
TRIM52 probe	5q35		193	ACCGATCCCAGAGAGAGGCTGTGAGTCT	GGATCTGGGCGTTCGCACCCTGAGAGAGGGGGTCAAAGCTC
Control probe	Y		118	GTTTATTCTAACCTAGGCAAACGGCATGCT	ATCACAAGAAAGGTTTAAAGCTTTGATAAAATGGGGGAGATTT
Control probe	5q31		130	ACATTGTCACTGCAAATCGACACCTAT	TAATGGGTCTCACCTCCCAACTGCTTCCCCCT
Control probe	4q11		136	GCGTAAGAGCAAAAAGCGAAGGCGCAA	TCTGGACACTGGGAGATTCGGAGCGCAGGGAGTTTGAGAG
Control probe	6p21		154	CTGAAACTCAGCACCGAGGCTCT	GCTCCCCACTCTGAGAGTGATCTACCAGAGCAGGAAGA
Control probe	3p22		172	GGCATCCAGATGGTGTGTGAGACGTT	GACTGAGTGCTGGGACCACGACCCAGAGGCCCGTC
Control probe	10q22		220	GCAGGCCACAGAGATGCTGGT	TCACAATGCCCAGAACCTCATGCAGTCTGTGAAGGAGACT
Control probe	16q24		256	CAGCATATTCAGGAGGCCTTACTACGT	GTCCCACTTCCTCCCCGCCCTGCTCACACCTCGAGTG
Control probe	16q22		274	GCCTCCGTTTCTGGAATCCAAGCA	GAATTGCTCACATTTCCCAACTCCTCTCCTGGCCTCAGAAGAC
Control probe	3p25		310	GAGCAAAGCCACTGAGGTATCTCTACAAA	ACCCACCAGAGTCTGGCACTGATGGTTGCATTTTGTTAATT
Control probe	3q12		328	GAAGACGCTGTCCATTTTCACAGAACTCT	GAAGGAGGCTTGTGACCAGCATGGTCCAGATCTCTACC
Control probe	5q33		382	TTGGTATCCGGATGCCCCTGG	AGAAATGGTGGTCCTCACCTGTGACACCCCTGAA
Control probe	2p14		409	GTTGTTGACCTCCTGTACTGGAGAGACAT	TAAGAAGACTGGAGTGGTGTTTGGTGCCAGCCTATTCCTGC
Control probe	1p36		436	CCTGCACAGATGGCGGCTATC	AGGCCCTTCCGGTTTTCCTGCTCCTTCAGTATCTCCT
Control probe	13q14		463	GGCCCTAGAGTGGGAGTCCTGATAA	CCCAGGCCTGTCTGACTACTTTGCCTTCTTTTGTAGCATATAGG