Table 1

Primers, polymerase chain reaction (PCR) product sizes, annealing temperatures, and high throughput denaturing high performance liquid chromatography (HT-DHPLC) analysis conditions for all the exons of the CAPN3 gene

Exon	Primers 5′–3′		Product size (bp)	PCR temperature (°C)	DHPLC temperature (°C)
Exon	Forward	Reverse	Product size (bp)	PCR temperature (°C)	DHPLC temperature (°C)
New primers are indicated in bold.
Prom	Ttcagtacctcccgttcacc	gatgcttgagccaggaaaac	296	60	58.5
1A	Ctttccttgaaggtagctgtat	cggctgatgatggctgaatagatgc	210	60	64.5
1B	gaggctgggggtggaaacccaagtg	gaggtgctgagtgagaggac	279	60	59
2	Actccgtctcaaaaaaatacct	attgtccctttacctcctgg	239	60	58.5
3	Ctcacgatctgtgccctgtgtc	cgctggccctcttaactacactctc	185	60	61.4
4	ggacacatttcctaacagtaatttgagt	ctagccccacctcacattctaaag	227	60	61.7
5	Gtgaccccaaattggtcttcat	ggtaaaaggcttcctgacacttg	262	60	60.7
6	ccctgtgttgttccctacattct	ggagggatggtcagttctgata	220	58	59.5
7	aatgggttctctggttactgc	cttcatgaggttaacccaggag	169	60	63
8	acagaagattccctttccagaga	atcttcaatactctcatggctcagt	239	60	63.5
9	cctgcttccttaattcctccatttt	ctcttccccacccttacccttct	159	60	60.3
10	cctcctcacctgctcccata	tttttcggcttagaccctcc	252	60	64
11	gtgaatgcgtgcttcctttctg	aaagtatgtttcctgggctggag	242	60	62.7
12	attcacatctgaagcatcttcctt	gtagtgctgcacacaactgctg	93	60	56.3
13	tgtggcaggacaggacgttc	ttcaacctctggagtgggcc	337	60	63
14	gggttctctagaggctggttct	gacttttcgcacagaagatctaaac	191	60	62
15	tcacttattctgcatttactgtttcc	agtacacacacttcatgctctctacc	94	60	52.4
16	cagactgtaatcctcccttccttcctg	tgtagcgtgctggccaccccac	205	60	62.1
17	acctctgacccctgtgaacc	gcgtcctgggcttggaggtac	146	58	60
18	tcctgaaccatgaccctcctct	ctgctttgagacaacgggactc	154	60	60
19	tcctcactcttctccatccc	accctgtatgttgccttgg	159	60	62.5
20	gtgtgctgtgtagccctgacctc	cttgactcccacgcttcattttct	134	60	60.3
21	gaatggggttgatttggagattcag	cacatccctgccaccccttc	170	60	61.4
22	ctccacgtccacctctaacatgg	agagggcagcccccctctccca	179	60	61.4
23	tctggcctgtgcattctttcac	ccctccaccttgagttttaatg	137	60	58.5
24	gggtcactcttttctgatctaca	agggtgaaactgaaatcctgag	131	60	60