Oligonucleotide primers for SDHB, SDHC, and SDHD sequencing analysis
| Gene | Exon | Forward primer | Reverse primer | Amplicon length (bp) | ||||
|---|---|---|---|---|---|---|---|---|
| SDHB | 1 | CGCCCACCCGGGAA | GTGGCTTTCCTGACTTTTCCC | 272 | ||||
| 2 | GTATTTCTAATTTTTTTTTCCTTTTTGTGA | TCTTCTCCAATAGCTGGCTTTCA | 211 | |||||
| 3 | GCTAATACATCCAGGTGTCTCCG | CCCAACAGGAATGAAATGCTC | 201 | |||||
| 4 | GGACTGATTCCGGATATGGGT | AATAGCGTAACACACATAGCACTGC | 256 | |||||
| 5 | GAGTAGTCAGTGTCCAAGAAATGGG | CTTATGTTCCCTGCCAAGCC | 301 | |||||
| 6 | TCTCTCCCGTCACAAGCTCC | AAACCAGGCCCCTCAGAATG | 251 | |||||
| 7 | GAGCTTTGAGTTGAGCCAGGG | CTTCTGGCGTGTCAGCTCTG | 251 | |||||
| 8 | CTGAGGAAGGAGTTTCACCCAA | TCCCTGCGGCAAGTAAAGG | 351 | |||||
| SDHC | 1 | AACCAGCAAACCAGCTAGGC | AACAGTTATCAGCAAAACGTGAGG | 263 | ||||
| 2 | ATCCCTTCACCCCTAAAAATAGAGA | GCTTGCAGTGAGCCGAGATC | 251 | |||||
| 3 | GCCTGGCTTGGTATTGCAAA | TCTGGCTCCAGAATCCTTCC | 230 | |||||
| 4 | CTATTTCAGAATTAGTTTATATTTTTGCCAA | GCTGAGTTTCAAAGGAGGCG | 251 | |||||
| 5 | TAACTTATGAGCAGCTGTGACAAGC | CCACTCCCTTCACAGAGAAAATGT | 253 | |||||
| 6 | GGAACTGTTAATGTCCTATTTACTGAAAT | TTTCCCAGGCTGGAGATAAGAAT | 251 | |||||
| SDHD | 1 | TGACCTTGAGCCCTCAGGAACG | TCAGGGTGGGAAGACCCCT | 191 | ||||
| 2 | GATCATCCTAATGACTCTTTTCC | AGCAGCAGCGATGGAGAGAA | 252 | |||||
| 3 | CTTTTATGAATCTGGTCCTTTTTTG | CAACTATATTTGGAATTGCTATAC | 338 | |||||
| 4 | AGTGGAGTGGCAAATGGAGA | AATGGCATGACAAAGCAGAGG | 360 |