Table 2

Primers used for sequencing of CDH3

Exon	Primer name	Primer sequence	Length of PCR fragment (bp)	Annealing temperatures (°C)
1	CDH3-EX1-F	GACCAATCAGCACCCACCT	384	67
1	CDH3-EX1-R	GAGGGCAGAGAGTGAAGGAG	384	67
2	CDH3-EX2-F	CAAGGGAGTCCCGGAAGG	277	60
2	CDH3-EX2-R	GGTCCACACCAAAATGGTCA	277	60
3	CDH3-EX3-F	GTCAGAGGACTCTTGTCAGTCTTG	248	60
3	CDH3-EX3-R	GCTCCTGGCCAGCAATTT	248	60
4	CDH3-EX4-F	AGGTGAGAGGATGTTGAGCA	284	65
4	CDH3-EX4-R	AAATCTCATGCAGGCCTTTG	284	65
5, 6	CDH3-EX56-F	GCCCCTCTTCACAGAGGACT	581	65
5, 6	CDH3-EX56-R	TGATACACAGCCAAGGAAATCA	581	65
7	CDH3-EX7-F	GTAGACAGGGCTGGAGTTGG	353	68
7	CDH3-EX7-R	ACGTGGGTCCTCACTGTTCT	353	68
8	CDH3-EX8-F	CAGTGCTTCCTGGAGGTCAG	286	65
8	CDH3-EX8-R	AGACTGGTTCCAGTTTCCTGAG	286	65
9	CDH3-EX9-F	GTTGGATGGAGGCTTCTCAG	360	65
9	CDH3-EX9-R	GGACTAGCCCACGGTAGACA	360	65
10	CDH3-EX10-F	CACATCTCAACTGTCCTGCAC	392	65
10	CDH3-EX10-R	GAACAAACGTTGGCCATGAT	392	65
11	CDH3-EX11-F	TCTGCCAGTTGGTATGAGGA	311	60
11	CDH3-EX11-R	AGGAAACATGCTGTGCTGTG	311	60
12	CDH3-EX12-F	AGAGCTGGGCGGTAAACAG	389	65
12	CDH3-EX12-R	GTGTGCAGAATCCTGGTGTG	389	65
13	CDH3-EX13-F	TGTGGAAGCCGTATTCTCAA	394	60
13	CDH3-EX13-R	TGGTGGCCTCTTAGTTCCAG	394	60
14	CDH3-EX14-F	CTGCAGTTAGAGGGGCTCTG	374	65
14	CDH3-EX14-R	ACCAGAGAAGTGCTGGAGGA	374	65
15	CDH3-EX15-F	TCCCCATGAGCCAGAGTATC	390	65
15	CDH3-EX15-R	GTCAAAAGCTGGTTGGTGGT	390	65
16	CDH3-EX16-F	GAGAGAGGGGCTCACAGAGA	595	65
16	CDH3-EX16-R	GGACACAGGGTTGAGCATTT	595	65