Primer sequences and conditions for direct sequencing (polymerase chain reaction; Paris cohort)
| SNP | Primer sequence (5′–3′) | Annealing temp (°C) | |
|---|---|---|---|
| Forward | Reverse | ||
| CFH, complement factor H; MCP, membrane cofactor protein; PCR, polymerase chain reaction; SNP, single nucleotide polymorphism; temp, temperature. | |||
| CFH −257 C→T | GGGGTTTTCTGGGATGTAATA | GTGATTAGTGCAGGAAAGAAC | 60 |
| CFH c.2089 A→G | TTGATCAAATGCTTGCCTCAG | TATATCTCCACAGGTACTCTC | 60 |
| CFH c.2881 G→T | TAGACAGACAGACACCAGAA | ACCACTTACACTTTGAATGA | 57 |
| MCP −547A→G | GCAAAGGGCAAATTACCTTAG | ACCCCTCAGGGTTAGTTTTAT | 62 |
| MCP −261A→G | ATAAAACTAACCCTGAGGGGT | CCTTTTTTCTTGCTAAGCCCT | 60 |
| MCP c.IVS8+23 T→G | CCAAGTGGTTGATCTTCTAAC | ATGGCTATACAAATGTCCTCC | 60 |
| MCP c.IVS9−78 G→A | GGGGAGGAAGAAGAAAGATTA | CTATGTTTGGGCACCTCATAA | 60 |
| MCP c. 2232 T→C | GTGTTCGGTGATTTCAGAAAG | TAAGGAGGGAGAGAAAAACAC | 55 |