Table 1

Primers used for quantitative real time polymerase chain reaction of the SALL4 gene as well as of the genomic region 100 kb 5′ to the ATG and 100 kb 3′ to the stop codon.

Primer name	Position	Sequence 5′-3′	Amplicon length
Position numbers refer to the 220 kb region as described in Methods.
10F	10656–10682	CCTTTTCCAACACCCATCATCCCAGCA	230
10R	10885–10860	CCAAGTCACCCTCACTCTCCTCACCA	230
16F	16234–16257	CATGGGGGTGGAGGAGGCTGGAGA	163
16R	16396–16373	AGGGGGTGGAGGGAAGGAGAAAGA	163
41F	41734–41760	GCCCAGGGACCTACTTCCGTCTTGCCA	150
41R	41883–41857	TTCTGAGCCCCTCGCTTTCTCACTGCC	150
49F	48877–48901	AAGGTGTGGGGGGGCAGTGGAGCAG	161
49R	49037–49011	TGGAGAGAATGAGGCAGCCAGTGGGGG	161
65F	65489–65513	GGCACAGTGGAAAGTCTCCCCCAAC	113
65R	65601–65579	GCTAGGCGGTGGCTCACACACAA	113
81F	81354–81376	TCCCCAGTCCCCCAAATCCCACA	233
81R	81586–81563	GGGGAGGAGGAGCAGAAGCAGTGA	233
Ex1-F	101935–101958 exon 1	GAGTTGAGATGGGTGGGAGGGCTG	206
Ex1-R	102118–102141 exon 1	GATGTCCACCAGCCGAGAAGGGAG	206
104F	104044–104069 intron 1	GGGGTTTCCCGAGAAGACGGTCGCCA	175
104R	104218–104194 intron 1	AAACGCCCAGCCATCCCCTCCGTCA	175
111F	111150–111175 intron 1	TCCCCTGACCCACCCCCAGCCTACAC	150
111R	111300–111274 intron 1	ACTTTTGACAGCACAGTCCACCCACGG	150
Ex2-F	113309–113330 exon 2	GGCAGGGGCCGACACTCTGAAG	254
Ex2-R	113543–113563 exon 2	GGAAGCAAAGCGCTCGGGAGG	254
Ex3-F	115957–115980 exon 3	GTTCCTGGCACATTTGTGGGACCC	244
Ex3-R	116178–116201 exon 3	GCTGAAAGCCCACACAAACCCACC	244
Ex4-F	120368–120392 exon 4	CACACGGGGCGAACAATAACTCAGC	250
Ex4-R	120594–120618 exon 4	CAAGGAAACCGGGAGGGTAGGAACC	250
135F	134925–134952	GGTGGCTCCATCCTGTGTGACAGCAAA	142
135R	135067–135042	TCACCAGAAGCCTTTCCCTGCTCCC	142
146F	146514–146540	GGTTTTTGGAGGCAAGGCATGGAACCG	164
146R	146678–146652	CCCAACCCCTCCACCCCTTCAAAGGCA	164
161F	161653–161679	TCTTGCAGCCCATCTCCCCAGCCTGTC	178
161R	161830–161805	TGCCCCAGGGTTCCAGAGGAGCAGTG	178
172F	172750–172775	TGAGGTGAGCGGTCCAGCGAGGGACG	199
172R	172948–172923	ACCCCCACTCCCAGGTCACCCTCCCA	199
175F	175123–175149	GGCACCCCGAGAAGAGAGACCAGAGGT	100
175R	175221–175195	CCCTGCCTGCACGTACCCCAGGAAGAA	100
194F	194058–194082	GCAACCAACAAAAGCCCCATCGGAG	284
194R	194341–194316	GCCGCAAAGAGAGACCCACAAAGGAA	284
217F	217607–217633	GCAAGGAGAGGGAATGAAGCAGCACAA	296
217R	217902–217879	GGAAAGTGGCAAGGAGGGGCAGGA	296