Exon | Forward primer | Reverse primer | Product length (bp) | PCR annealing temp (°C) | DHPLC analysis | |
---|---|---|---|---|---|---|
Temp of elution (°C) | Buffer (%) | |||||
2 | AGGTAAGATTGGTTACTAGAG | AATAACTAACTGTTCATTGCTC | 350 | 58 | 55 | 57 |
57 | 57 | |||||
3 | TGAGAAGCAATGTCAGTATAG | CCTGTACATGCCCACACGC | 201 | 58 | 55 | 53 |
4 | CTGGCTTCTTGCTACTCTG | TCCCACTTCTTCCACACTC | 242 | 58 | 54 | 53 |
5 | AGTGTTCTTGGAATTCTTTG | GGCAAATGTGCACATTGGC | 244 | 54 | 57 | 53 |
6 | CTCTGTATTGGATGAATTATTC | TTCTTAAAGACAGTAACATGTG | 303 | 59 | 54 | 55 |
56 | 55 | |||||
7 | TTTATCTTGACACTCCAGA | ACTCCTCCAGCAATCAATT | 237 | 56 | 53 | 53 |
8 | GCCCATCGGAGAACAGTCATTAC | GCAAATGACAATAGAATCAGCAG | 280 | 55 | 56 | 54 |
57 | 54 | |||||
9 | TTATTCACTTGTGTTCTGATGAT | CAAATACTGCAGTATTGATTCC | 410 | 54 | 54 | 58 |
56 | 58 | |||||
10 | TATGAATTTGACTGGGATTGG | CTATGGTCACATGTAGACAC | 275 | 59 | 57 | 54 |
11 | TTGATATTCTGCAATGACTGTG | AGCCACCTCCTCCACCTAC | 333 | 62 | 53 | 56 |
55 | 56 | |||||
12_a | TTGTGTGTCAGCTATTGAGG | GGTTCTGCTGAGATCTGCTG | 406 | 60 | 56 | 60 |
59 | 60 | |||||
12_b | CAACAACATACCACACCTTC | TTCTCGTGTCACTGTGTCTG | 422 | 60 | 57 | 60 |
60 | 60 | |||||
12_c | ACTCCAAGTCTGTGAGCAAC | AGATGGACCCTCATCACATC | 541 | 60 | 57 | 63 |
13 | GGTTATGGTCCTAGTTCTAC | CACTTCAACTTATTTGTGGG | 298 | 60 | 57 | 55 |
59 | 55 | |||||
14 | CAATAGAGTGAGACCCTGTC | CTGAGTCGGTGAAAGCAGTG | 279 | 65 | 57 | 55 |
60 | 55 | |||||
15 | AAAAGTCCATCAGTGACTTAC | CCTAGCAGGAGAAAGGACAC | 262 | 60 | 56 | 54 |
16 | TATAGGAACCTAGTGTCATGC | CAACTTTGATTGCCAAGTGC | 293 | 59 | 53 | 57 |
17 | CTTGGGTGTGGTTGCATATC | CTGTAACATTGAGAGGCTAAG | 296 | 60 | 59 | 55 |
18 | CTTGCACATGCTTGCCCTTC | CACCCAGCTGTTCAGAGTAG | 418 | 62 | 61 | 58 |
19 | ACTCTGGTCAATGGGATGTG | CATTCAGTAGTCTAGGGTCG | 249 | 60 | 59 | 53 |
20 | TTGGCTTCAGCTGGTGTCTG | CATCTGCATTTCTACAGCTC | 345 | 61 | 61 | 58 |
21 | CATTAGCCAGAGTGCACCTG | AGGAAAAACTCAACCTCAGCG | 290 | 60 | 59 | 55 |
63 | 54 |