Table 1

RAI1 PCR primers and annealing temperatures*

Exon	Forward primer	Reverse primer	Product size (bp)	Annealing temp
Primers cover the coding region of RAI1* and are based on Genbank No AY172136.
†Amplification requires Invitrogen® 10× PCR enhancer.
PCR, polymerase chain reaction; temp, temperature; UTR, untranslated region.
3	SHE 323: TCTGAGGCAAAAGGAAGTGG	SHE 324: GACTGGGAATGCTGAGGAAA	770	62°C
3	SHE 325: AAAGGCAGAAGCTGCAGAAC	SHE 326: CAGTGCTGGCTTTATGCTG	875	62°C
3	SHE 327: TGTCAAGAACCTCGTGTCCA	SHE 328: GGGAAACAGTCAAAAGCTGC	777	60°C
3	SHE 329: TCTCGCTGGAGAACCACAG	SHE 330: AACCAGCTCTGGACCTTTGA	904	59°C
3	SHE 331: CACTCATCTGCACCAAGGAG	SHE 332: ATGAAGGCCGACTTCCTTTT	1000	60°C
3	SHE 333: CCAAGAAGCTCCTCGACAAC	SHE 334: TTCCGGCTCCTCTTCTTAGG	763	59°C
3	SHE 335: AAACCGAGGCCTTCACATC	SHE 336: CACACTCGGGACCTTTGAGT	964	60°C
3	SHE 339: CTACCCTGAACACTGCCTCC	SHE 340: AGAGGCAATGGAGACAGGAA	887	60°C
4	SHE 341: CCAGGCTGGTATCAAACTCC	SHE 342: GGCAAGAGAGGAGGGAAACT	420	60°C
5	RA 46: GGAGTGGAGTGGAGTGTGGAGG	RA 45: GGACTGTGAAGGAGGTGCGAGG	310	62°C
6+3′ UTR	SHE 345: ACTGTGAAGTCCGAGGTCGT	SHE 346: GACTGGAAAGGGGACAAACA	816	55°C†