Primers and PCR conditions for direct sequencing of coding region of ZNF81
| Exon | Forward (5′–3′) | Reverse (5′–3′) | Tann | [MgCl2] |
|---|---|---|---|---|
| 3 | GTTCAGCGCTAAGTACCATTGA | GAGCTAATTGCAAATTACGAAG | 60°C | 1.5 |
| 4 | CTCCATCCTCCAGTGCTGATCATG | GGGCACAATTTAACCCATAACAGG | 60°C | 1.5 |
| 5 | CTCAGTTCCCCATGAAATGTGG | CCAACCTTTAAGCAATAACAGC | 60°C | 1.5 |
| 6a | CAGCTTTCTCAGACTGTACCCC | CGTGGTGACTATAAGAAGAGTTC | 60°C | 1.5 |
| 6b | GACTTACATATTCATAATAAAAGC | GACTTAGAAGGGAAAGATTTCCC | 60°C | 1.5 |
| 6c | CCTATATCTGTACTCAATGTGGG | GTTAGACCTGACTTTTGTCAGCC | 60°C | 3.0 |