Primers to amplify KLK4
| Exon | Sequence | Annealing temperature, °C | Product size, bp |
|---|---|---|---|
| 1 | F 5′- TGGAAGTTTGCAGCACCTGA –3′ | 62 | 227 |
| R 5′- GCGACATGGAGCTGTAGGGA –3′ | |||
| 2 | F 5′- CTCATCCCCAGGTGCTGACGT –3′ | 58 | 222 |
| R 5′- GCCATGGGGGACGGATAACA –3′ | |||
| 3 and 4 | F 5′- GCCCCCAGCCCTGACTCG –3′ | 62 | 1195 |
| R 5′-TCACGCACTGCAGCACGGTA –3′ | |||
| 5 | F 5′- GAGGTCCTCTGCCCAGTCG –3′ | 58 | 550 |
| R 5′-ATTGGTAAGGCTCCCCGTCA –3′ | |||
| 6 (set 1) | F 5′- TATGTCGCTGGGCTATGTTG-3′ | 58 | 281 |
| R 5′- GGCCTGGACGGTTTTCTC-3′ | |||
| 6 (set 2) | F 5′- TGGCGTGCCAGGTGTCTA –3′ | 62 | 761 |
| R 5′- ATGGAAGGGGAAGCAAGGAG –3′ |