Conditions for PCR amplification of genes involved in cytochrome oxidase biogenesis
| Gene | Fragment | Reaction | Forward primer | Reverse primer | Annealing | Size |
|---|---|---|---|---|---|---|
| SURF 1 (9 exons) | Exons 1–2 | gDNA-PCR | 5′ tcgtgacctgtcaggatgc | 5′ cagacagcaggtggctctg | 56°C | 736 bp |
| Exons 3–9 | RT-PCR | 5′ aggacgtcctcagggtc | 5′ catgatccagcataaaggca | 61°C | 897 bp | |
| COX10 (7 exons) | Exons 1–5 | RT-PCR | 5′ gtcccgtgaggagagaggac | 5′ ccaaggtcagaatggcaact | 62°C | 817 bp |
| Exons 6–7 | RT-PCR | 5′ ttttgaggtgccatttgactc | 5′ aacccagcaatttctcttcttg | 60°C | 797 bp | |
| SCO1 (6 exons) | Exon 1 | gDNA-PCR | 5′ gatggacagagcgactcctt | 5′ aactgggactacccgcaag | 61°C | 552 bp |
| Exons 2–6 | RT-PCR | 5′ agtacccggacgagttatgc | 5′ tgcgagacagtttccttcct | 60°C | 948 bp | |
| SCO2 (2 exons) | Exons 1–2 | RT-PCR | 5′ gcttcctctcgtgcttggt | 5′ taaacgcagcccgtttaatg | 61°C | 901 bp |
| COX15.1 (9 exons) | Exons 1–6 | RT-PCR | 5′ ttgtggaagaggtggctgtt | 5′ agacctgctgttccatgagc | 58°C | 842 bp |
| Exons 5–9 | RT-PCR | 5′ gacgtgttcttgccctctgt | 5′ ttgaccatttggaaaccactt | 808 bp | ||
| Exon 5 | gDNA-PCR | 5′ caagatcccgccactg | 5′ gtccccatttaacgaacaat | 60°C | 598 bp |