Table 1

Primers for mutation detection and polymerase chain reaction amplification conditions

Exon	Forward primer (5′→3′)	Reverse primer (5′→3′)	Annealing temperature (°C)
Note: the coding sequence of PHF8 begins at exon 2 and ends at exon 22.
1	tcaagtcacttggcttctcga	atgagaggaggtgaactcggt	62
2	actgagggttggtgcaagg	gagagcaagtgaacacacctg	63
3	acagcactgttctagacctca	cctttcctttgtctctcctct	56
4	gctcaggcctttgttcttggt	gggaaagctgggaagaggt	63
5	gtggcagcctccaataag	aaacagattggaggggaagg	59
6	ggactgatgaggggacaagaga	agagaaacacgaatatacactag	59
7	gctgtggggtattttattcaggc	gattcaaaatgtttctgtgctgc	59
8	gttttggagcatgggagaaa	ttaatatgctgtggggccaa	60
9	ttgagatgagtaggtagtggaga	ccttctacattatactcctcact	58
10	catccagaacaaaacctgattc	tatcatgtcttgactgcgttac	59
11	gttgtcttaacaaaagtccca	ctctccaaaacatctaccca	60
12	tctccattgaattgccttgg	atgctaactgcagaggcctaa	58
13	aggaacagacctctgctgtta	ggatagcctgctttttgaaac	59
14	ccagtgaaaccagagggaact	ctgtctcagtggcatattacc	59
15	cttgctagcgactaacaatac	tccgtctcaaaacaaacaaaca	62
16	Gtatttggctgaaatgctaatc	ccaactagaatgacaatctgtc	59
17	tcatcgcccctgtactggg	ctgattggctgacctggcac	64
18	gatgaccgatttactctgacag	gatggagactgggactgagg	64
19	tcattctatcttcaccctttg	catccttgtctatttcctcct	59
20	agagcttggaggtagggaat	tctaagtcaaactgctattagt	59
21	gtctagttagttcctttcctgg	taagatccttcggttctacaacc	59
22	ttggtgccagatttgatgttg	gaaggcaggcaggatgctcta	63