Exon | Forward primer (5′→3′) | Reverse primer (5′→3′) | Annealing temperature (°C) | |
---|---|---|---|---|
Note: the coding sequence of PHF8 begins at exon 2 and ends at exon 22. | ||||
1 | tcaagtcacttggcttctcga | atgagaggaggtgaactcggt | 62 | |
2 | actgagggttggtgcaagg | gagagcaagtgaacacacctg | 63 | |
3 | acagcactgttctagacctca | cctttcctttgtctctcctct | 56 | |
4 | gctcaggcctttgttcttggt | gggaaagctgggaagaggt | 63 | |
5 | gtggcagcctccaataag | aaacagattggaggggaagg | 59 | |
6 | ggactgatgaggggacaagaga | agagaaacacgaatatacactag | 59 | |
7 | gctgtggggtattttattcaggc | gattcaaaatgtttctgtgctgc | 59 | |
8 | gttttggagcatgggagaaa | ttaatatgctgtggggccaa | 60 | |
9 | ttgagatgagtaggtagtggaga | ccttctacattatactcctcact | 58 | |
10 | catccagaacaaaacctgattc | tatcatgtcttgactgcgttac | 59 | |
11 | gttgtcttaacaaaagtccca | ctctccaaaacatctaccca | 60 | |
12 | tctccattgaattgccttgg | atgctaactgcagaggcctaa | 58 | |
13 | aggaacagacctctgctgtta | ggatagcctgctttttgaaac | 59 | |
14 | ccagtgaaaccagagggaact | ctgtctcagtggcatattacc | 59 | |
15 | cttgctagcgactaacaatac | tccgtctcaaaacaaacaaaca | 62 | |
16 | Gtatttggctgaaatgctaatc | ccaactagaatgacaatctgtc | 59 | |
17 | tcatcgcccctgtactggg | ctgattggctgacctggcac | 64 | |
18 | gatgaccgatttactctgacag | gatggagactgggactgagg | 64 | |
19 | tcattctatcttcaccctttg | catccttgtctatttcctcct | 59 | |
20 | agagcttggaggtagggaat | tctaagtcaaactgctattagt | 59 | |
21 | gtctagttagttcctttcctgg | taagatccttcggttctacaacc | 59 | |
22 | ttggtgccagatttgatgttg | gaaggcaggcaggatgctcta | 63 |