Oligonucleotide sequences used for the generation of FISH probes
| Probe | Oligonucleotide pairs | Annealing °C, PCR enhancers if needed and product size | |
|---|---|---|---|
| Forward (5′–3′) | Reverse (5′–3′) | ||
| “D22S427” | A: ccaaagcgttgggattac | A: cacccatgtgtctcagatgc | 56°C, 486 bp |
| B: gacccacgagagctgaagac | B: gggtctcagccttcagacag | 56°C+10% DMSO, 477 bp | |
| C: cccgttcatgatttatgtgg | C: cgatctgagctcactgcaac | 56°C, 476 bp | |
| D: gcctggctgcattttacatt | D: gactcggctctcggtcctac | 56°C, 448 bp | |
| E: gagtcgaaggactttgggagt | E: ggagggtgctgacaaaactg | 56°C, 494 bp | |
| ZNF74 | A: attgggggtagcagctcttt | A: gggcaacatctggactcttc | 56°C, 479 bp |
| B: tagctcactgcagccttcaa | B: gtggttaccaaggggagagg | 56°C, 425 bp | |
| C: gttccacatgcccttgaaaa | C: caagagccaaactctgtcaaaa | 56°C, 499 bp | |
| D: ggatttgggttccagtctga | D: ccagaacaggtctcagcaca | 56°C, 453 bp | |
| E: gtgttgttggccctattgct | E: gttagggaggccagttaggg | 56°C, 448 bp | |
| “D22S936” | A: caccccttcactgcttgagt | A: agattgtgccactccactcc | 56°C, 455 bp |
| B: ctgcagagccacttttggtt | B: aatcaaagccctgaatgtgg | 56°C, 429 bp | |
| C: gcccacccaggataatcttc | C: gggcaacaagagcaaaactc | 56°C, 477 bp | |
| D: caggggttaatttggaagga | D: agctcagctctggctctgac | 56°C, 500 bp | |
| E: taaagaaccagcctcggtgt | E: tggtggtggaagagaaggac | 56°C, 480 bp | |