PCR amplicons and primers to detect sequence variation in the AMPD1 locus: primers for exons, upstream, downstream, and introns
| Region | Primer Name | Primer sequences (5′ to 3′) | Length(bp) |
|---|---|---|---|
| Ex, exon; (FAM)-, ; (HEX)-, ; (ROX)-, ; (NED)-, Up, upstream; Pro, promoter; Int, intron; Fl, flanking; STR, short tandem repeat. | |||
| Ex1 and upstream | pro 1 (+) | (FAM)-AGTAAAGACTGAAAGCTTCAC | 282 |
| int 1 (-) | (HEX)-CAACTGAGCTCTCCAAACAC | ||
| Ex 2 | int 1 (+) | (ROX)-GCAATCTACATGTGTCTACC | 197 |
| int 2 (-) | (NED)-TTCTGAATTAAGGAATAGTCTC | ||
| Ex 3 | int 2 (+) | (FAM)-AGCAGAAACTCTCAGCTGAC | 291 |
| int 3 (-) | (HEX)-CTCCTTAGGTGCCAATATAC | ||
| Ex 4 | int 3 (+) | (ROX)-TCACAATGAAACGCCTGATG | 261 |
| int 4 (-) | (NED)-CTGGTTGCTAAGTCTGAGTG | ||
| Ex 5 | int 4 (+) | (FAM)-AATGGGCTGCGATACACAG | 369 |
| int 5 (-) | (HEX)-CTGGATTACTTATAATCTAGAG | ||
| Ex 6 | int 5 (+) | (ROX)-CAAGCTAATGAGATGTTGTAG | 371 |
| int 6 (-) | (NED)-GTTTAGGTCTTGTAGTACTAG | ||
| Ex 7 | int 6 (+) | (FAM)-CTATGAATGATAAAGGTCCTG | 262 |
| int 7 (-) | (HEX)-TGCCCAGGAATTACCCCTG | ||
| Ex 8 | int 7 (+) | (ROX)-CAGCACCATATCCTCTAAAC | 315 |
| int 8 (-) | (NED)-TTCTGAGTATTAGAGTGAAAC | ||
| Ex 9 | int 8 (+) | (FAM)-AGACCCCAGTTCTGCTTGAC | 267 |
| int 9 (-) | (HEX)-TCAAGAACCATGCCAGATAC | ||
| Ex 10 | int 9 (+) | (NED)-GAGTTAGAAAGGGAATCTCC | 296 |
| int 10 (-) | (NED)-GATGAGACAAGGGCAGGC | ||
| Ex 11 | int 10 (+) | (FAM)-ATTCTCTGGTTCCCTCATAC | 285 |
| int 11 (-) | (HEX)-GCACCAAGAATTAGTCATGAC | ||
| Ex 12 | int 11 (+) | (ROX)-TTGGTCTGTGCCTTTGGAC | 305 |
| int 12 (-) | (NED)-AAAGAGCTGTGTCAAATAGAC | ||
| Ex 13 | int 12 (+) | (FAM)-GCTTACTTCCTTCCAGAGTG | 261 |
| int 13 (-) | (HEX)-TAGCTCCAATGTAAAAGTTAAG | ||
| Ex 14 | int 13 (+) | (ROX)-CATGAGATTGTAGTGTTTCTAG | 304 |
| int 14 (-) | (NED)-GACGGGAAACAACAGTCCAG | ||
| Ex 15 | int 14 (+) | (FAM)-TTTCAGGCAGGATGCATATTA | 221 |
| int 15 (-) | (HEX)-CAGACCCTATACTCAGAAGG | ||
| Ex 16 | int 15 (+) | (ROX)-ATTATTGTCTATACCAGGGTG | 306 |
| int 16 (-) | (NED)-AGTAGGGACTCAATAACAGG | ||
| Up1 | us 31 (+) | CCATAACTTCAGATGAGCAC | 326 |
| us 31 (-) | ACAGAACTGAATGCTTACTG | ||
| Up2 | us 11 (+) | AAAGAACTTTTGGAGACATCC | 340 |
| us 11 (-) | TTACATTTTTACCTGGATTTCC | ||
| Pro4 | pro4 (+) | GCAGCAACTATCAAATATTTAG | 427 |
| pro4 (-) | CCCAAATCTCACGCTCTTTC | ||
| Pro3 | pro3 (+) | CAGTCATAACTAAGACTGTGTG | 417 |
| pro3 (-) | GCACACACGCTCAGGAATG | ||
| Pro2 | pro2 (+) | CTGCTAAGTCAGACACATTAC | 349 |
| pro2 (-) | GCTTGAGGCATTTGATAGTG | ||
| Int2 | int 2 (+) | TCTGCCTCCTGGGTTCAAG | 371 |
| int 2 (-) | AGGAAAGACAAGGTTGTAGG | ||
| Int4 | int 4_10kbB+ | CCAGACGCTTTTGGCCAAG | 413 |
| int 4_10kbB- | AAACCACTCGGTCAGTGAG | ||
| Int6 | int 6 (+) LD | AGCCTTTGGAGCCCACTAC | 423 |
| int 7 (-) LD | TAAAATCCACTGAACCTGTAG | ||
| 3′Fl | ds 60 (+) | GCGGATGGTAACAATAACAAC | 331 |
| ds 60 (-) | GAAATAGTTTTTTATAGACCTC | ||
| Int2STR | int 1 (+) | (ROX)-GCAATCTACATGTGTCTACC | 437–457 |
| int 2 STR(-) | CCTGGGTGACCATCCATC | ||
| Int9STR | int 8 (+) | (FAM)-AGACCCCAGTTCTGCTTGAC | 379–406 |
| int 9 STR (-) | TGTTTGGCCCCAGGGATC | ||
| 3′STR | STR 1D (+) | (HEX)-TGAGTTTGCCAGGCAGAAAC | 222–236 |
| STR 1D (-) | AACTTAAGACTCACTACAGAC | ||