Table 1

Primer sequences, PCR conditions, temperatures, and temperature gradients used for DHPLC mutation screening of genes K8 and K18

Exon	Sequence	MgCl₂	Anealing temp	Product size	Temp gradient
Temp, temperature.
K8 Ex1 5′	5′-AGAAGCAGCTTCTCCGCTC-3′
K8 Ex1 3′	5′-GGACTACCAGGAGAAAGGGG-3′	2.0 mM	64.1°C	452	62.5–65.5°C
K8 Ex2 5′	5′- AAAAGACTGCTTGCTTTCCTC -3′
K8 Ex2 3′	5′- AGGTGACTTCAGTTGGGTGG -3′	1.5 mM	64.1°C	322	60.0–62.0°C
K8 Ex3 5′	5′- AGGGCAACCTCCCTTTTG -3′
K8 Ex3 3′	5′- CAGTGTCCAGATAGGAGAAGGG -3′	2.0 mM	64.1°C	148	59.5+60.5°C
K8 Ex4 5′	5′- GGCTCCCCTCTCCTCATC -3′
K8 Ex4 3′	5′- TCTCCTGCGACCAGGAAC -3′	2.0 mM	62.7°C	142	60.0+61.0°C
K8 Ex5 5′	5′- ACCACTTGCCCTCTTCCC -3′
K8 Ex5 3′	5′- AGCTGAACTGTGGCTGCC -3′	1.5 mM	65.6°C	458	60.0–62.0°C
K8 Ex6 5′	5′- GAAGAGAAGGCATACCCAACC -3′
K8 Ex6 3′	5′- ACAGGACCCCAAGTCCAAG -3′	1.5 mM	65.6°C	373	63.5–65.5°C
K8 Ex7 5′	5′- GAAGGGCTAGGAGTTGGTCC -3′
K8 Ex7 3′	5′- TGAGCGACTGAGCACAGC -3′	1.5 mM	65.6°C	188	61.0–66.0°C
K8 Ex8 5′	5′- TGTGGCTACCTCTGTCCCTC -3′
K8 Ex8 3′	5′- GAGGGGTAGGCTGGGAGG -3′	1.5 mM	62.7°C	246	64.0–66.0°C
K18 Ex1 5′	5′- CCTGTCCTTTCTCTCTCCCC -3′
K18 Ex1 3′	5′- TCCCTCCTACCCCTTACCTG -3′	1.5 mM	61.3°C	461	62.0–67.0°C
K18 Ex2 5′	5′- TGGGACCAGGAAGTTTTCAC -3′
K18 Ex2 3′	5′- GTCCTTCTTAGCTCCACCCC -3′	1.5 mM	62.7°C	241	60.0+61.0°C
K18 Ex3 5′	5′- TGGATCAGAGATAGGGGCC -3′
K18 Ex3 3′	5′- TTCTCTTGGCCCCTCAATC -3′	1.5 mM	62.7°C	248	62.0+63.0°C
K18 Ex4 5′	5′- AGAGGCAATCACAGAAGAAAGG -3′
K18 Ex4 3′	5′- CACACCTTGGCAGCCATC -3′	1.5 mM	62.7°C	276	60.5–63.5°C
K18 Ex5 5′	5′- TCTCAGCAGGTGCGTGAG -3′
K18 Ex5 3′	5′- GGGCAGGTGATGTGAAGG -3′	1.5 mM	62.7°C	245	61.5–63.5°C
K18 Ex6 5′	5′- GATGAGCAGTCCTGGGACTC -3′
K18 Ex6 3′	5′- CAGTGAACAGACACATGGGG -3′	1.5 mM	62.7°C	377	62.0–64.0°C
K18 Ex7 5′	5′- GCTGTTTATAACTTGGGCTTGG -3′
K18 Ex7 3′	5′- TCCTGCTCCCAAAGGGTAC -3′	1.5 mM	62.7°C	190	61.0+62.0°C