PCR primers and conditions
| Test | Gene ID | PCR; [Mg]; DMSO | F primer | R primer | S primer |
|---|---|---|---|---|---|
| Gene ID refers to HUGO names for all the query genes. PCR refers to the PCR conditions used: A indicates that Amersham Biosciences and E indicates that Eurogentec PCR buffers and Taq polymerase were used. 3 or 1.5 indicates the final concentration of MgCl2 and 5% indicates the final concentration (v/v) of DMSO. b at the start of the primers indicates 5′biotinylated primer. F, R, and S refer to forward, reverse, and sequencing primers, respectively. | |||||
| Hsa 21a | ITSN | A; 3 | ATTTATTGCCATGTACACTT | bGAATCTTTAAGCCTCACATAG | ACCAAGAAAGATGGTGAC |
| Hsa 21b | GABPA | A; 3 | bCTTACTGATAAGGACGCTC | CTCATAGTTCATCGTAGGCT | TCACCAACCCAAGAAA |
| Hsa 13a | NUFIP1 | E; 1.5 | bGCTGAGCCGACTAGTGATT | AAGGGAAGCGAGGACGTAA | GGAAGCGAGGACGTA |
| Hsa 13b | STK24 | A; 1.5 | CGCTCTCGTCTGACATTT | bTCAGACATTTTTAGGTGG | CATTTGTTTGGAATCGT |
| Hsa 18a | KIAA1328 | A; 3; 5% | CGAAGGAAATGTCAGATCAA | bGACTCCATGGAGATTGAAG | TGTCAGATCAAGACACA |
| Hsa 18b | WBP11 | A; 3 | bGGAGGGACGGGAAGTAGAG | GTGAAGAAGCAGTGGATGTGCC | CAGAATCATCTTCATCAT |
| Hs XYa | ARSD | E; 3 | CGCCAGCAATGGATAC | bTGCAAAAGTGGTTTCGTTC | GGCCCTTCAGTGGA |
| Hs XYb | TGIF2LX | E; 3 | bAAGACAGCCCGGCGAAGA | ATTCCGGGAGAATGCGTCTGC | TGATAAACCAGTTAGAAATC |
| Hs XAa | TAF9L | E; 3 | bTGCCTAATGTTTTGTGATT | GACCCAAAACTACCTGTC | GTAAAACCCAACTG |
| Hs XAb | JM5 | E; 3 | CCCTGTGTGTCTCTAAACCAGC | bGGTGGCAGGGTCAGT | GAAACTGGTGGAGCTG |