Table 1

Details of primers used to sequence VEGFR-3 and conditions for amplification

Exon	Forward primer	Reverse primer	Annealing temperature (°C)	Magnesium concentration (mmol/l)
1	ccgcgcggacactttcagcc	gtacccggcggagcggtctc	64	1.5
2	cgatgcagaatgcccagccatg	cacagccaccacctcctgcc	66	1.5
3	ctggtgaggctcggcctgag	agcccagggtccacaggctg	65	1
4	tggctggttcaggaccagcac	ccttgctcctggccagacag	64	1.5
5	ctgtctggccaggagcaagg	caggacgacacccactgagg	64	1.5
6	gggctgtcactgcctcactgg	cagtgtgtgctgtacagtcacag	65	1.5
7	gacgcggggcttccctg	cacaaggaccctggtttcc	61	1
8	cctggtcagtccacgacggg	tctccgtgtgcaggtcctgac	65	1.5
9	gggccctgacctcccttc	cccgtgcgctctcccgtc	59	1
10	cagctgaccggccatctg	ggggacctgaggctggag	61	1
11	ctgcccttgcctcttctg	gggcactcagcagcgcg	61	1
12	gcacagcgacctccttgcag	ctcaatgagcccaaacctgcc	65	1.5
13	tgccacagggcttcttcc	gtccccgccctccccg	61	1
14	gtcctcctgctaagtgcagctc	gcctcgcgggcctccggac	65	1.5
15	gtccggaggcccgcgaggc	cttctggtggccacgacttgc	65	1
16	gcaagtcgtggccaccagaag	gaactagggcgggcaccttattc	65	1.5
17	ctgtcctgggcaaagttctgcc	atggagggattcaggcactccg	65	1.5
18	tgtctccacgctcaccc	ccgctgaccccacacctt	55	1
19	tgccttagctaagcggccagtg	gctccgcgtttgcacccgcg	65	1
20	cgcgggtgcaaacgcggagc	cgcagaggcgcctccattcc	65	1
21	gccctcgagccagcttcg	aggaaaagggaagaggccag	61	1
22	acatgcttgttagctgttccctg	caagcacttcttggcgactggt	63	1.5
23	gagttgacctcccaaggt	tctcctggacaggcagtc	56	1.5
24	ttcaacccaggcagaggaccc	cgtaacctgccgccagtgacc	64	1.5
25	caggacggggtgacttga	gcccaggcctgtctactg	56	1
26	gggcactgaaggtcctgggac	gcagctcgggccagggacc	67	1
27	gctgagggcagtggctggtg	aaatgcaccttgtccacatggc	65	1
28	cctcagcaccttctgatttc	cccacccttcatgtgaagt	55	1.5
29	ggtggctgccctgcaggagg	ggagggcaacatcgatacctgc	65	1.5
30	tactaacaccaccttccctg	gtgctgggggtcttgtcc	60	1.5