Oligonucleotide primers used for screening the CLCA1 gene polymorphisms
| Name | Sequence | Position | Size (bp) | AT (°C) |
|---|---|---|---|---|
| AT, annealing temperature; bp, base pairs. | ||||
| CL.pro1.F | 5′-AGAGTGAATCTGACCAGCTC-3′ | −1614 to −1595 | 328 | 61 |
| CL.pro1.R | 5′-GAGAGAGGAAGGAAAAGCGT-3′ | −1288 to −1307 | ||
| CL.pro2.F | 5′-TTTCTAAGCGACTGCCGAAAG-3 | −1394 to −1374 | 354 | 58 |
| CL.pro2.R | 5′-CCCTAAAAAACAGAGCCTA-3′ | −1042 to −1062 | ||
| CL.pro3.F | 5′-AACTCTCAAACTCTTAGCAC-3′ | −1094 to −1073 | 230 | 58 |
| CL.pro3.R | 5′-GCAGTTAAAAGGGAAACACT-3′ | −866 to −885 | ||
| CL.pro4.F | 5′-TGGATCATCTGGACAACCAC-3′ | −933 to −914 | 319 | 61 |
| CL.pro4.R | 5′-GCAATTGTGAATGGGAGTTC-3′ | −616 to −635 | ||
| CL.pro5.F | 5′-ACAGACAAACAGAGAGCC-3′ | −663 to −646 | 350 | 48 |
| CL.pro5.R | 5′-TTGGTCTTCAGATGGTGA-3′ | −315 to −332 | ||
| CL.pro6.F | 5′-CAATGCCTTTCTTCACAGA-3′ | −402 to −384 | 412 | 58 |
| CL.pro6.R | 5′-GCATTAGCAAGTGATGTAGA-3′ | +9 to −11 | ||
| CL.ex1.F | 5′-TGAGGAACTTGGAGTATGC-3′ | −88 to −70 | 217 | 59 |
| CL.ex1.R | 5′-CTCCTGGTGGTCTTTACC-3′ | +129 to +112 | ||
| CL.ex2A.F | 5′-GTGCTTCCAAGGAACCGGATG-3′ | +300 to +320 | 291 | 63 |
| CL.ex2A.R | 5′-CTGTGATTCCTCTCTTTGG-3′ | +590 to +572 | ||
| CL.ex2B.F | 5′-ACCTTCGTAACCCGCATTT-3′ | +552 to +570 | 282 | 58 |
| CL.ex2B.R | 5′-CTCATGTTCTCTCTATCAC-3′ | +833 to +815 | ||
| CL.ex3.F | 5′-TCAGTCAATTGTTACGTATG-3′ | +5012 to +5031 | 221 | 55 |
| CL.ex3.R | 5′-CAGGAGAAAGTGGAATTT-3′ | +5232 to +5215 | ||
| CL.ex4.F | 5′-GAAACTTACTCCAGTACGA-3′ | +5284 to +5302 | 256 | 55 |
| CL.ex4.R | 5′-AGAGTATGAGAAGTATGGT-3′ | +5539 to +5521 | ||
| CL.ex5.F | 5′-TCCAGTGTGAGAAGGTAA-3′ | +8014 to +8031 | 203 | 55 |
| CL.ex5.R | 5′-GTTTGGAACAAGTTAAATCA-3′ | +8216 to +8197 | ||
| CL.ex6.F | 5′-CACCTAAACATCTAACCTTCC-3′ | +13805 to +13825 | 232 | 57 |
| CL.ex6.R | 5′-GGGGTGAGAACAAGGTACTTA-3′ | +14036 to +14016 | ||
| CL.ex7.F | 5′-TTCTACCACATAGTTTACCATT-3′ | +16924 to +16945 | 293 | 58 |
| CL.ex7.R | 5′-AAGACCAAGACTCATTGAACA-3′ | +17216 to +17196 | ||
| CL.ex8.F | 5′-CCATCTAAACGTAACCTGTTT-3′ | +18124 to +18144 | 291 | 58 |
| CL.ex8.R | 5′-CAATAGAAAAACACATCTCAC-3′ | +18414 to +18394 | ||
| CL.ex9.F | 5′-TTTGGCCACCCTAAGTCTGA-3′ | +20588 to +20607 | 266 | 63 |
| CL.ex9.R | 5′-GTAGGAGAGACAATACCGGG-3′ | +20834 to +20853 | ||
| CL.ex10.F | 5′-GCTCTCTAAACAACAGCTAA-3′ | +22827 to +22846 | 243 | 59 |
| CL.ex10.R | 5′-CTCCATCTTGGCTATGATTA-3′ | +23069 to +23050 | ||
| CL.ex11.F | 5′-CTCCAGAAAGTAAGAGCTG-3′ | +24995 to +25014 | 292 | 54 |
| CL.ex11.R | 5′-TTATCTTGTTGAATTATGAGA-3′ | +25286 to +25266 | ||
| CL.ex12.F | 5′-GAAGTTATTCATTGGAAATGT-3′ | +25790 to +25810 | 335 | 52 |
| CL.ex12.R | 5′-AAATAAATATCTTCCAATTTC-3′ | +26124 to +26104 | ||
| CL.ex13.F | 5′-CCCGTTACCTATTTATTAATTC-3′ | +27110 to +27131 | 247 | 58 |
| CL.ex13.R | 5′-TTTGCACAAGCCAGGTATGTT-3′ | +28953 to +28974 | ||
| CL.ex14.F | 5′-ACCATTTAGTGATGTTGCTTA-3′ | +30174 to +30194 | 337 | 58 |
| CL.ex14.R | 5′-TCTACTTGGCTGGTAAATCT-3′ | +30510 to +30491 | ||
| CL.ex15A.F | 5′-CAGAATTAAAGTCACATTCTT-3′ | +31266 to +31286 | 345 | 56 |
| CL.ex15A.R | 5′-GAATATTAGGACAAGGAGC-3′ | +31610 to +31592 | ||
| CL.ex15B.F | 5′-CAGAGACACCTAGTCCCG-3′ | +31542 to +31560 | 383 | 56 |
| CL.ex15B.R | 5′-TAAATTTTTGGGTTTGAATGG-3′ | +31945 to +31925 | ||