Spontaneous and mutagen induced instability of the mononucleotide repeat BAT-25 and the myotonic dystrophy type 1 (CTG) and fragile X syndrome (CGG) trinucleotide repeats in multiple single cell clones of MMR proficient (SW480 and HCT116+chr.3) and deficient (HCT116 and LoVo) cell lines
| Treatment | Marker | Cell line | |||
|---|---|---|---|---|---|
| SW480 | LoVo | HCT116 | HCT116+ch3 | ||
| MC/TC (%) | MC/TC (%) | MC/TC (%) | MC/TC (%) | ||
| MC/TC (%), mutant clones/total clones (percentage of mutant clones). | |||||
| The difference between control and MMC treated cells is statistically significant with **p<0.01 or ***p<0.001. | |||||
| Control | BAT-25 | 6/39 (15.4) | 25/35 (71.4) | 11/33 (33.3) | 0/40 (0.0) |
| CTG | 1/39 (2.6) | 0/36 (0.0) | 3/32 (9.4) | 1/40 (2.5) | |
| CGG | 0/41 (0.0) | 6/57 (10.5) | 1/44 (2.3) | 0/40 (0.0) | |
| MMC | BAT-25 | 15/40 (37.5) | 27/38 (71.0) | 23/40 (57.5) | 0/40 (0.0) |
| CTG | 18/40 (45.0)*** | 1/40 (2.5) | 8/40 (20.0) | 3/40 (7.5) | |
| CGG | 21/39 (53.8)*** | 2/40 (5.0) | 10/40 (25.0)** | 0/40 (0.0) | |