Primer sequences, conditions, and expected product sizes for human genomic and cDNA PCR amplification used for SNP analysis of CP genes
| Genomic DNA PCR: | RT-PCR: | |||||
|---|---|---|---|---|---|---|
| CPA genes | Primer sequences (5′-3′) | Expected size (bp) | Conditions | Primer sequences (5′-3′) | Expected size (bp) | Conditions |
| CPA1 | F:tgagaacttctggcaccaac | 401 | 54°C for 32 cycles | F:aggtacagaaggtgaaggag | 540 | 54°C for 35 cycles |
| R:taccagccatgatgaagagc | R:tgtccaaggtgtcgagaatg | |||||
| CPA2 | F:ttaacattgcaggagaccgg | 320 | 54°C for 32 cycles | F:agtgggttacacaagctacg | 198 | 55°C for 32 cycles |
| R:cacatctccttaggtccttg | R:agaggcttccagataccttg | |||||
| CPA4 | F:gagcgtcttgcatttctgtg | 353 | 54°C for 32 cycles | F:caacccttgctccgaagtgt | 359 | 56°C for 35 cycles |
| R:tccctataaagctgcagagc | R:cataggtcccggtatctctc | |||||
| CPA5 Iso 1 | F:tggctgccatagtgaacttc | 181 | 58°C for 32 cycles | F:gcaacagctttgaaaaccag | 607 | 60°C for 48 cycles |
| R:tttcctgtgaagcggtctc | R:ccaaaaatgtactcgatccc | |||||
| CPA5 Iso 3 | F:tgcaaggttcctaagcctag | 406 | 60°C for 32 cycles | F:cgggttttggagatgtggcc | 185 | 64°C for 48 cycles |
| R:cagggtgtgctccatgatgg | R:cagggtgtgctccatgatgg | |||||