Primer pair sequences and PCR running conditions for genetic markers analysed
| Locus | Primer ID | Primer sequence (5′-3′) | Primer annealing temperature (°C) |
|---|---|---|---|
| D8S1778 | AFMb327yh1 | F:ttttgcaggagggcgtcta | 60 |
| R:ccactgagcccagcctatta | |||
| D8S559 | AFM352td9 | F:aattgaagtgaggtaggaggttg | 61.5 |
| R:agctattgctcttacaggaggg | |||
| D8S1762 | AFMb307xb5 | F:gtgtaatccagttccccag | 55 |
| R:ttgctgtaaacctttggc | |||
| CA1 | F:agagccaacctcccaaaact | 62 | |
| R:gctcatatttaaacaccactcgg | |||
| CA2 | F:cagtgtcctggctaccatgt | 59 | |
| R:atggcctaccctgtgattgt | |||
| CA3 | F:ccaagagaaacatactcattacca | 59 | |
| R:aagaaagagcaaaaagggca | |||
| D8S1714 | AFMa1842zg5 | F:cccttgccagagccat | 60 |
| R:ccactgacgccctgat | |||
| D8S521 | AFM078za9 | F:tttgaaatcctacagagttcct | 60 |
| R:ctgttaatgaatgcggtgtc |