Table 1

Effects of missense mutations in the MBD on transcriptional repressive activity

	Relative transcriptional activity (%)*
	pGL-SNRPN		pGL-LS5
Mutation	Methylated	Unmethylated	Methylated	Unmethylated
*Relative transcriptional activities (%) compared with that of only pAc5.1/V5-His vector are presented.
Vector	100	100	100	100
wt	3.2 ± 0.3	56.1 ± 4.3	14.2 ± 1.6	59.6 ± 6.5
D97E	11.9 ± 2.4	85.3 ± 5.6	36.8 ± 2.1	102 ± 13.1
D97Y	15.1 ± 1.9	90.8 ± 10.5	35.3 ± 3.1	107 ± 6.4
L100V	3.8 ± 0.5	65.1 ± 3.2	15.7 ± 0.8	86.9 ± 7.1
P101H	2.4 ± 0.3	25.8 ± 3.3	10.1 ± 1.2	77.7 ± 5.8
P101L	3.8 ± 0.5	58.7 ± 8.1	12.9 ± 2.5	75.3 ± 3.8
P101R	1.9 ± 0.2	39.3 ± 4.9	12.1 ± 0.9	72.9 ± 4.5
P101S	1.8 ± 0.3	28.4 ± 3.6	13.9 ± 2.6	80.8 ± 6.6
P101T	2.2 ± 0.1	29.2 ± 4.1	14.2 ± 0.2	63.2 ± 8.1
R106Q	57.7 ± 4.8	126 ± 9.6	85.3 ± 9.8	117 ± 10.5
R106W	61.2 ± 7.6	137 ± 12.5	78.6 ± 5.1	126 ± 15.1
R111G	127 ± 11.2	119 ± 8.8	139 ± 10.8	123 ± 7.2
Y120D	13.7 ± 2.3	87.2 ± 10.6	33.1 ± 1.6	106 ± 11.8
L124F	22.4 ± 3.6	98.6 ± 3.7	34.8 ± 2.9	119 ± 13.9
P127L	2.3 ± 0.1	26.4 ± 4.1	13.1 ± 1.5	74.1 ± 4.8
R133C	2.1 ± 0.2	23.8 ± 3.7	14.4 ± 1.6	57.6 ± 8.1
R133H	2.3 ± 0.1	30.4 ± 1.8	13.9 ± 1.8	100 ± 7.9
R133L	13.6 ± 2.2	64.3 ± 5.6	42.3 ± 3.9	97.7 ± 8.8
S134C	2.3 ± 0.3	28.1 ± 2.1	11.2 ± 2.8	74.2 ± 9.5
K135E	12.9 ± 1.8	90.8 ± 8.4	27.2 ± 4.1	121 ± 10.6
E137G	19.2 ± 2.5	96.1 ± 7.9	31.8 ± 2.8	78.7 ± 6.2
A140V	2.6 ± 0.3	23.9 ± 4.6	11.7 ± 0.8	60.8 ± 5.1
P152R	6.1 ± 0.9	67.3 ± 8.7	16.4 ± 2.9	84.1 ± 6.6
F155I	3.1 ± 0.2	59.2 ± 7.5	16.6 ± 0.8	86.7 ± 10.2
F155S	57.8 ± 7.8	120 ± 8.8	55.3 ± 3.6	90.1 ± 4.9
D156E	38.3 ± 5.9	105 ± 7.5	50.7 ± 7.6	107 ± 8.1
D156G	37.1 ± 0.6	109 ± 11.8	49.2 ± 3.1	119 ± 10.9
T158A	5.8 ± 0.7	75.9 ± 6.2	17.9 ± 2.7	85.4 ± 8.8
T158M	17.3 ± 2.1	69.3 ± 4.1	32.1 ± 2.9	81.3 ± 6.7