Relative transcriptional activity (%)* | ||||
---|---|---|---|---|
pGL-SNRPN | pGL-LS5 | |||
Mutation | Methylated | Unmethylated | Methylated | Unmethylated |
*Relative transcriptional activities (%) compared with that of only pAc5.1/V5-His vector are presented. | ||||
Vector | 100 | 100 | 100 | 100 |
wt | 3.2 ± 0.3 | 56.1 ± 4.3 | 14.2 ± 1.6 | 59.6 ± 6.5 |
D97E | 11.9 ± 2.4 | 85.3 ± 5.6 | 36.8 ± 2.1 | 102 ± 13.1 |
D97Y | 15.1 ± 1.9 | 90.8 ± 10.5 | 35.3 ± 3.1 | 107 ± 6.4 |
L100V | 3.8 ± 0.5 | 65.1 ± 3.2 | 15.7 ± 0.8 | 86.9 ± 7.1 |
P101H | 2.4 ± 0.3 | 25.8 ± 3.3 | 10.1 ± 1.2 | 77.7 ± 5.8 |
P101L | 3.8 ± 0.5 | 58.7 ± 8.1 | 12.9 ± 2.5 | 75.3 ± 3.8 |
P101R | 1.9 ± 0.2 | 39.3 ± 4.9 | 12.1 ± 0.9 | 72.9 ± 4.5 |
P101S | 1.8 ± 0.3 | 28.4 ± 3.6 | 13.9 ± 2.6 | 80.8 ± 6.6 |
P101T | 2.2 ± 0.1 | 29.2 ± 4.1 | 14.2 ± 0.2 | 63.2 ± 8.1 |
R106Q | 57.7 ± 4.8 | 126 ± 9.6 | 85.3 ± 9.8 | 117 ± 10.5 |
R106W | 61.2 ± 7.6 | 137 ± 12.5 | 78.6 ± 5.1 | 126 ± 15.1 |
R111G | 127 ± 11.2 | 119 ± 8.8 | 139 ± 10.8 | 123 ± 7.2 |
Y120D | 13.7 ± 2.3 | 87.2 ± 10.6 | 33.1 ± 1.6 | 106 ± 11.8 |
L124F | 22.4 ± 3.6 | 98.6 ± 3.7 | 34.8 ± 2.9 | 119 ± 13.9 |
P127L | 2.3 ± 0.1 | 26.4 ± 4.1 | 13.1 ± 1.5 | 74.1 ± 4.8 |
R133C | 2.1 ± 0.2 | 23.8 ± 3.7 | 14.4 ± 1.6 | 57.6 ± 8.1 |
R133H | 2.3 ± 0.1 | 30.4 ± 1.8 | 13.9 ± 1.8 | 100 ± 7.9 |
R133L | 13.6 ± 2.2 | 64.3 ± 5.6 | 42.3 ± 3.9 | 97.7 ± 8.8 |
S134C | 2.3 ± 0.3 | 28.1 ± 2.1 | 11.2 ± 2.8 | 74.2 ± 9.5 |
K135E | 12.9 ± 1.8 | 90.8 ± 8.4 | 27.2 ± 4.1 | 121 ± 10.6 |
E137G | 19.2 ± 2.5 | 96.1 ± 7.9 | 31.8 ± 2.8 | 78.7 ± 6.2 |
A140V | 2.6 ± 0.3 | 23.9 ± 4.6 | 11.7 ± 0.8 | 60.8 ± 5.1 |
P152R | 6.1 ± 0.9 | 67.3 ± 8.7 | 16.4 ± 2.9 | 84.1 ± 6.6 |
F155I | 3.1 ± 0.2 | 59.2 ± 7.5 | 16.6 ± 0.8 | 86.7 ± 10.2 |
F155S | 57.8 ± 7.8 | 120 ± 8.8 | 55.3 ± 3.6 | 90.1 ± 4.9 |
D156E | 38.3 ± 5.9 | 105 ± 7.5 | 50.7 ± 7.6 | 107 ± 8.1 |
D156G | 37.1 ± 0.6 | 109 ± 11.8 | 49.2 ± 3.1 | 119 ± 10.9 |
T158A | 5.8 ± 0.7 | 75.9 ± 6.2 | 17.9 ± 2.7 | 85.4 ± 8.8 |
T158M | 17.3 ± 2.1 | 69.3 ± 4.1 | 32.1 ± 2.9 | 81.3 ± 6.7 |