Summary of potential splice site acceptors in the region of intron 9/exon 9, respective CVs, and translation properties of corresponding mRNAs
| Acceptor site | Sequence (AG position) | CV splice view | CV10 | CV branch* | In frame | Premature stop (position) |
|---|---|---|---|---|---|---|
| *Branch site considered was the one with highest CV. Position of branch alanine is indicated between brackets and is relative to the new exon border (see Methods). | ||||||
| †Not recognised as potential splice acceptor (CV below 72). | ||||||
| Int 9-Alt1 | TTTTTCTAG TTTG | 89 | 78.51 | 74.79 | No | Yes |
| (1524 + 63/64) | (−20 nt) | (+39 nt) | ||||
| Int 9-Alt2 | AGTTTGTAG TGCT | 85 | 73.48 | 74.79 | Yes | Yes |
| (1524 + 70/71) | (−27 nt) | (+39 nt) | ||||
| Int 9-Alt3 | TATTTTCAG TGC | 84 | 75.41 | 72.65 | No | Yes |
| (1524 + 199/200) | (−37 nt) | (+72 nt) | ||||
| Int 9-Alt4 | CTTAACCAG AACA | 82 | 70.09 | 67.15 | No | Yes |
| (1524 + 299/300) | (−28 nt) | (+36 nt) | ||||
| Int 9-Alt5 | TGTGCATAG CAGA | 81 | 70.18 | 70.97 | No | Yes |
| (1525-164/5) | (−27 nt) | (+9 nt) | ||||
| Int 9-norm | TATTTCCAG ACTT | 81 | 72.12 | 59.81 | Yes | No |
| (1525-1/2) | (−37 nt) | |||||
| Ex 10-Alt1 | TGTTCTCAG TTTT | 83 | 71.15 | 66.94 | Yes | No |
| (1610-1611) | (−19 nt) | |||||
| Ex 10-Alt2 | TGAATATAG ATAC | † | 61.18 | 63.02 | No | Yes |
| (1678-1679) | (−29 nt) | (+213 nt) | ||||
| Int 10-norm | TGGTAATAG GACA | 83 | 72.02 | 65.60 | Yes | No |
| (1714-1/2) | (−20 nt) | |||||