Table 1

Oligonucleotide primers, MgCl₂ concentrations, and annealing temperatures for FH PCR. DHPLC melting temperatures correspond to column temperatures used in heteroduplex analysis. Mitochondrial targeting signal is defined as exon 0 (FH0)

Exon	Primer	Primer sequence (5‘ > 3‘)	Product size (bp)	MgCl₂ (mmol/l)	Annealing temp (°C)	DHPLC melting temp (°C)
0	FH0F	TGT GAG GCT GTT GAT TGG AT	299	0	59	66
	FH0R	GGA GGG CTG AAG GTC ACT G
1	FH1F	AAG ATG CGA TTA CTT TTG ATC C	256	2.8	58	58
	FH1R	TGA ATA CAG CCT ACT TCA TCCA A
2	FH2F	CTG CCA AAA TAA TAA ACT TCC ATG C	254	1.4	58	53/56
	FH2R	GCC AGA GCA TAT CGT CAT CC
3	FH3F	TGT GGG TCA ACT GTA TTC AAA C	321	1.4	58	57
	FH3R	CAA TCT CAG GTA TGC TTT TCA A
4	FH4F	GCT GGG TTT TGA GTA GTT AGT TGG	349	1.4	58	56/57
	FH4R	GGC CAT TTG TAC CAA GCT CT
5	FH5F	TTTGCTCATCATAAGATTTGAAGT	381	2.8	60	59
	FH5R	CAGACCACGTATAATGAGAAATGAA
6	FH6F	GTT CAC CCA TCT AGG ATA TTT TTC T	340	2.8	60	61
	FH6R	GCT TCG GGA TAA CTT TAA ACA AA
7	FH7F	ATGGTTGGGCCTTGCTTTAT	300	2.8	58	54/57
	FH7R	CCAAGATAATAAGCCTTTGGTCA
8	FH8F	CATGTTGCCTTAGTAATGTCTCTCTC	317	1.4	58	56
	FH8R	TGCTGTTCTCAAACACTGATCC
9	FH9F	CAATTATGTCACCTTTTGCTTTAGG	312	2.8	58	53/56
	FH9R	GCAGTTTCCTTTCAAACTTATCC