Table 1

Primer, forward and reverse primer sequences, and annealing temperatures for PCR for primers used to amplify candidate genes exons 1–7 for GSTA2 and exons 1–13 for MAN1A2

Primer	Sequence	Tanneal
GSTA2 1F	GTA TTG TTT AAA CTT TGA TTA	53.5
GSTA2 1R	GTG CTA AGG ACA CAT ATT AGT
GSTA2 2F	TCA TTC ATT CAT TCA TTC AC	53.5
GSTA2 2R	AAC GCA ATC GTA AAT CTG
GSTA2 3F	GAT AGA GGC CAT TCA ATT	53.5
GSTA2 3R	AGA TAC CCT CAT TAG AGA
GSTA2 4NF	CAA ATT TAG TCA TTT CAA CAA CCA	61.0
GSTA2 4NR	GTC CCA CCC ACT CAA GGA AGG
GSTA2 5CF	GAC CAC CGA TGA TCT TC	53.0
GSTA2 5CR	ATA ATT CCA TAT TGG GGT T
GSTA2 6NF	TTC CTG ATC TGA TTC CTC CCA TTC	64.7
GSTA2 6R	ATG TGG GTC TGC CTC TCT GAA
GSTA2 7F	CCC TTG ACT TGT TGT TTT AG	53.5
GSTA2 7R	CTT GAC CTC TAT GGC TGG
MAN1A2 1F	TTC TAA AGT ATT CTC TCC AAG AGC GTA AA	64.6
MAN1A2 1R	ACC GTA CCT TGC TCT GCC AAA T
MAN1A2 2F	TCC TGG GTT TAA TGG AGA AAC CTT	58.3
MAN1A22R	TCC ATA AAC ACT CCA TAT CAC AAA
MAN1A2 3F	ATC TGG AAG AAT GGG CAA GGA T	58.3
MAN1A2 3R	TGG AGG TAG AAA ATG GAA ATA G
MAN1A2 4F	CCC CCA AAG AGA ACA GGA ACC	64.6
MAN1A2 4R	GCA GTT AGA CAT TGT GTC TTT TCA TGG T
MAN1A2 5F	TTT TCC ACA GGG TGT TTG TGA AA	60.6
MAN1A2 5R	TCC CCT CCA AAA CAA ACA ATG C
MAN1A2 6F	TGC TCC CGT GTA CCC AGT ACC T	58.3
MAN1A2 6R	TTC TTC CAG AAT AAG AAT ACC CAC AT
MAN1A2 7F	TGG TCT TTT CCC AAT CCT GTG TC	60.6
MAN1A2 7R	TCT TTG GGC CAA TCT TAA AGG A
MAN1A2 8F	AAA ACC AAG CCT TGA TGA CAC T	64.6
MAN1A2 8R	CAA CAT CAA GCA AAG AGT TTT CAG A
MAN1A2 9F	TTG TCT TGT CTT CCC TTG CCT TT	58.3
MAN1A2 9R	ACA CTG TAC ATT CAA TCC ATT AAG
MAN1A2 10F	CCC AGG TTT TAT AAT GGC CAG A	58.3
MAN1A2 10R	TGC CTA TAT ATT CTC CTT CTT ATT GC
MAN1A2 11F	TCC TTG CAA ACA AAC ATA AAT GG	60.6
MAN1A2 11R	GGC ATC ATA GGA GGG CAT GAG T
MAN1A2 12F	TGA AAT TCT ACG GAA TTG CTT ATT TTG T	58.3
MAN1A2 12R	TGA TCA CAC AGC AAA ACC AGT CT
MAN1A2 13F	TGG TTT TCT GTG CCA TTA AAA GTG AG	64.6
MAN1A2 13R	AAA AGC CGC CCC TTC AAA CTA A