Information and the primer sequences for the studied SNPs in the APOE and TGF-β1 genes
| SNP | dbSNP* or reference | Polymorphism† | Distance from previous SNP (bp) | Location in gene | Primers (5′- 3′) |
|---|---|---|---|---|---|
| *SNP ID in the database dbSNP, www.ncbi.nlm.nih.gov/SNP | |||||
| †Bold faced letters are the minor alleles | |||||
| ‡SNP 713–8delC35. | |||||
| §Insertion (+) or deletion (−) of nucleotide C | |||||
| ¶SNP C861–20-T35 | |||||
| APOE gene | |||||
| SNP1 | rs440446 | G-C | — | Exon 1 | F: TCCCCAGGAGCCGGTGA |
| R: CCCCAAGCCCGACCCC | |||||
| SNP2 | rs769450 | G-A | 1277 | Intron 2 | F: CCTCAGGTGATCTGCCCGTTTC |
| R: ACTCCTGGGCTCAAGTGATCCTC | |||||
| SNP3 | rs429358 | T-C | 1497 | Exon 4 | F: CGGGCACGGCTGTCCAA |
| R: CGAGCATGGCCTGCACCTC | |||||
| SNP4 | rs7412 | C-T | 138 | Exon 4 | F: GCTGCGTAAGCGGCTCC |
| R: GCGGCCCTGTTCCACC | |||||
| TGF-β1 gene | |||||
| SNP5 | rs1800469 | C-T | — | Exon 1 | F: GGGCCCAGTTTCCCTATCTGTAAA |
| R: CTGGGCCACCGTCCTCATC | |||||
| SNP6‡ | Langdahl et al35 | +/−C§ | 12 354 | Intron 4 | F: CCACGCCCCACTTATCTATCCC |
| R: GGAAAGGCCGGTTCATGCCA | |||||
| SNP7 | rs1800472 | C-T | 83 | Exon 5 | F: CAGGCTACAAGGCTCACCTGAA |
| R: GGTTCACTACCGGCCGC | |||||
| SNP8¶ | Langdahl et al35 | C-T | 9644 | Intron 5 | F: GGCTTGTCTTAAGCATTGCGTGAAATTAA |
| R: GTACAGCTGCCGCACGC | |||||