Primers used for IPS analysis and sequencing of the TM4SF2 gene
| Exon | Forward primer | Reverse primer | Annealing temp (°C) | Product size (bp) | Restriction enzymes Product size (bp) |
|---|---|---|---|---|---|
| 1 | 5`-cccgccccgccccgcctcttc-3` | 5`-acccagcaccccgcgcctgtctcct-3` | 68 | 308 | |
| 2 | 5`-gcaagtaccgtcttattcacggaa-3` | 5`-tgtctgtgacctcaagacgaacc-3` | 58 | 398 | HaeIII |
| 205; 149; 44 | |||||
| 3 | 5`-gtgaggacttgtctaattcccgc-3` | 5`-ggaaaacagctagcctatatgcacc-3` | 65 | 283 | |
| 4 | 5`-cctccctggctacatttatacagaga-3` | 5`-ctgggccaacctacaggcagtc-3` | 65 | 301 | |
| 5 | 5`-gcttttgcctgtgtttctcttat-3` | 5`-gatctctttcccttgactactattt-3` | 65 | 371 | PvuII |
| 194; 177 | |||||
| 6 | 5`-aagtgtatgttgggagtgtttcgagt-3` | 5`-gagctttgaccccatctatcagc-3` | 60 | 292 | |
| 7 | 5`-cgcatatgtcaaaaatcacataccc-3` | 5`-actcctgcacatttgaagggtagtaa-3` | 60 | 265 |