Table 1

Primers for PCR amplification of exons in the human cone α-transducin gene, GNAT2²²

Exon	Primer pairs (sense/antisense) 5`-3` direction	Amplified fragment length (bp)	Annealing temperature (°C)	(MgCl₂) (mmol/l)
1	AGTTGAAGTAGGGAGTCTCA	350	55	1.50
	TCTCTGGCTCATCTTCCCAT
2	GTGGAAATCGAAAGCATAAG	120	52	1.50
	TCTTCACCCTATCTTGTCTT
3	AGCTAAAGACAGAGTGTCTG	210	55	1.50
	CTGCTTCCACCCTTAACCAC
4	TGTGAAGTTCTTAACCAGGT	240	55	1.00
	CTAGAAGATTGCTTAAGCAT
5	GTCTCTTAGCCTCGTCTGTG	220	55	1.50
	TGTATCCGAGATGCCCTAGG
6	GTATGTTGGGCATACCTATG	210	55	1.50
	TGTTCTACCAAAGCTGCTTG
7	ATTCTATAAGCCAAATCTGA	250	55	1.50
	AGTCTCTACTAAAAGGCATT
8	TCAGCAACTAACAAGGGTTC	270	55	1.50
	ATACCTGAGGAATGGTGAGG