Table 1

PCR and SSCP conditions for the detection of sequence variations in exons 5-10 of the human SREBP-2 gene

Exon	Amplicon size in base pairs	Intronic oligonucleotide primers with EcoRI cleavage sites at the 5` end (5`–3`)	[MgCl₂] conc (mmol/l)	PCR thermal cycling profile	Conditions of SSCP electrophoresis
SSCP, single strand confirmation polymorphism; F, forward; R, reverse
5	278	F: cggaattctattctagaccctggtgggc	3.5	Denaturing: 94°C/45 sec	75 V, 14 h
		R: cggaattcgtacccaccttggcgtctgt		Annealing: 58°C/30 sec
				Extension: 72°C/1 min
				Cycles: 30
6	202	F: cggaattctggtctcactgtgttttcactcatc	3.5	Denaturing: 94°C/45 sec	65 V, 14 h
		R: cggaattcgccagggctgacaagccttttctca		Annealing: 56°C/30 sec
				Extension: 72°C/1 min
				Cycles: 32
7	313	F: cggaattcaaaagcagatcattttcaccaggtgggg	5.0	Denaturing: 94°C/45 sec	74 V, 14 h
		R: cggaattcctgtgacctcccagctatcttcttaggggaa		Annealing: 57°C/30 sec
				Extension: 72°C/1 min
				Cycles: 32
8	284	F: cggaattccacctcgaggcccttttttggaggtcaa	2.5	Denaturing: 94°C/45 sec	72 V, 14 h
		R: cggaattccatagtggtccagccatgcccagg		Annealing: 60°C/30 sec
				Extension: 72°C/1 min
				Cycles: 30
9	268	F: cggaattcttccaaagagactctgctgagacga	3.5	Denaturing: 94°C/45 sec	74 V, 14 h
		R: cggaattcgggggaggatggggaagggcggaca		Annealing: 57°C/30 sec
				Extension: 72°C/1 min
				Cycles: 30
10	363	F: cggaattcgccagtgaccattaacaccttttga	1.8	Denaturing: 94°C/45 sec	80 V, 14 h
		R: cggaattctgcagcaagccagtcatcagcagct		Annealing: 59°C/30 sec
				Extension: 72°C/1 min
				Cycles: 30