5 | 278 | F: cggaattctattctagaccctggtgggc | 3.5 | Denaturing: 94°C/45 sec | 75 V, 14 h |
| | R: cggaattcgtacccaccttggcgtctgt | | Annealing: 58°C/30 sec | |
| | | | Extension: 72°C/1 min | |
| | | | Cycles: 30 | |
6 | 202 | F: cggaattctggtctcactgtgttttcactcatc | 3.5 | Denaturing: 94°C/45 sec | 65 V, 14 h |
| | R: cggaattcgccagggctgacaagccttttctca | | Annealing: 56°C/30 sec | |
| | | | Extension: 72°C/1 min | |
| | | | Cycles: 32 | |
7 | 313 | F: cggaattcaaaagcagatcattttcaccaggtgggg | 5.0 | Denaturing: 94°C/45 sec | 74 V, 14 h |
| | R: cggaattcctgtgacctcccagctatcttcttaggggaa | | Annealing: 57°C/30 sec | |
| | | | Extension: 72°C/1 min | |
| | | | Cycles: 32 | |
8 | 284 | F: cggaattccacctcgaggcccttttttggaggtcaa | 2.5 | Denaturing: 94°C/45 sec | 72 V, 14 h |
| | R: cggaattccatagtggtccagccatgcccagg | | Annealing: 60°C/30 sec | |
| | | | Extension: 72°C/1 min | |
| | | | Cycles: 30 | |
9 | 268 | F: cggaattcttccaaagagactctgctgagacga | 3.5 | Denaturing: 94°C/45 sec | 74 V, 14 h |
| | R: cggaattcgggggaggatggggaagggcggaca | | Annealing: 57°C/30 sec | |
| | | | Extension: 72°C/1 min | |
| | | | Cycles: 30 | |
10 | 363 | F: cggaattcgccagtgaccattaacaccttttga | 1.8 | Denaturing: 94°C/45 sec | 80 V, 14 h |
| | R: cggaattctgcagcaagccagtcatcagcagct | | Annealing: 59°C/30 sec | |
| | | | Extension: 72°C/1 min | |
| | | | Cycles: 30 | |