Primer (5′→3′) | Size (bp) | PCR conditions* | GenBank accession No | ||
---|---|---|---|---|---|
Exon | Forward | Reverse | Size (bp) | PCR conditions* | GenBank accession No |
SDenotes primers also used in sequencing reactions. Sequencing was performed with BigDye Terminator System from ABI. | |||||
*The standard PCR amplification for each exon contains: Taq (0.025 U/μl), 1 × PCRx enhancer buffer, 25 nmol/l each dNTP, and 1.5 mmol/l MgSO4. A=5% PCRx enhancer. B=1 × PCRx enhancer buffer, no PCRx enhancer. | |||||
Cycling conditions=95-5’ + 94-30”/56-30”/72-90” 35X + 72-10’. | |||||
02–03 | TCCTGCTCCAAATGACTGAGTTCT | TCAACCCAATGGAATGACCTCTTA | 888 | B | AY162963 |
04–05 | GGTGGAGGCTTGACATCATCAGAG | GGAATAGGGCTCAGATGGTCTTTG | 1493 | A | AY162963 |
04–05S | GCCCTGGCCTCATGTGTCAATGTG | GGGTCACAGGGACAGACAGACAAT | AY162963 | ||
04–05S | CGGCGGCTACTACGTCTACAACCT | GTAGCTGCCCACCACATTGACACA | AY162963 | ||
06 | ACCTCTGGACCTCAAGTAATCTGT | TGATGCCTACTGGCTGAGACAATC | 940 | A | AY162964 |
07 | ACCAGCAGATTTAGCTTTGAAGTC | GCTTGAACCAGGCAGTGCTTTGAC | 475 | A | AY162965 |
08 | AGCAGCATCCAGGCACTTGTCAGA | TGAGGCAGAAGAATCACTTGAACC | 711 | B | AY162967 |
08S | TCCAAAGACCCCCTCTGAATTCTA | AY162967 | |||
09 | ATTTGAATCCAGGAAGTCTGACTC | GGCAAGCCACTGAAGTTCTCTGAG | 612 | B | AY162968 |
10 | GAGCGGCTCAGAGAACTTCAGTGG | CCCGTGTCCTGTGTTACATTCATC | 529 | B | AY162968 |
11 | GAGCCCCTGATGGGTCTGAAGTAG | TCTGAGCCACTCTCCTTATTTAGA | 345 | B | AY162969 |
12 | TAGATTGGGCACTTCACAAGAATG | ACAGCAGAACCCAGTCTCACTGAG | 733 | B | AY162970 |