Primers, PCR conditions, and restriction enzymes used for the genotyping
| Exon | Primers sequence (5` > 3`) | Annealing temp (°C) | Mg conc (mmol/l) | Product size (bp) | Restriction enzyme | Size of fragments |
|---|---|---|---|---|---|---|
| 2 | Fwd: TCACTCACATCCCTACTTCC | 60 | 1.2 | 263 | EagI | 207+87 |
| Rv: CTTATGCGGACACTGAGC | ||||||
| 13 | Fwd: CTCTCTGTCTGAACTTGGGC | 65 | 1.2 | 238 | TaqI | 139+99 |
| Rv: TCACCCTGCAGCTGGCCTTA | ||||||
| 14 | Fwd: AAGACCCAAGCTGCCTGA | 66 | 1.5 | 328 | – | – |
| Rv: GTGGTGGGTCAGGGTGTGG | ||||||
| 15 | Fwd: GACTCGTGCTATTTTTCCTC | 59 | 1.5 | 235 | RsaI | 106+129 |
| Rv: TATCTTTCCTAGGCTTCCCAA |