Table 2

Restriction endonuclease digestion confirming base substitutions in the HFE gene

Polymorphism	Restriction enzyme	Restriction enzyme site	Restriction site Wild type	Restriction site Base change
G→A C282Y		RsaI		G t▾ac	Absent	Present
				C a▴tg
C→G H63D		BclI		t▾gat C a	Present	Absent
				acta G▴t
A→T S65C		HinfI		g▾A ntc	Present	Absent
				c T na▴g
T→C 4T→C		RsaI		G t▾ac	Absent	Present
				C a▴tg
T→C 116T→C		Sau96I		g▾g N cc	Absent	Present
				cc N g▴g