Previously identified mutations in the 12 positive controls (the M34T mutation has subsequently been found to be detected by this strategy)
| Mutation | No of controls |
| 35delG/35delG | 3 |
| 35delG/wild type | 2 |
| 167delT/167delT | 1 |
| 167delT/wild type | 1 |
| V27I/E114G | 1 |
| 35delG/313–326 14bp deletion | 2 |
| 313–326 14bp deletion/wild type | 2 |