Table 1

Composition of multiplex PCR panels used for linkage analysis in FHC pedigrees

Multiplex name (No PCR cycles)	Marker data				Primer concentration (pmol/μl)
Multiplex name (No PCR cycles)	Marker name (gene)	Length (bp)			Fluorescent dye	HZ index	No of alleles	Forward	Reverse
MP1 (35 cycles)	D11S1993, (MYBPC3)	224–245	HEX	75	8		0.2	0.2
MP1 (35 cycles)	D7S661, (FHC locus)	252–282	TET	76	10		0.4	0.4
MP2 (30 cycles)	D3S3685, (MYL3)	195–221	TET	89	13		0.2	0.2
	D3S3564, (MYL3)	204–220	HEX	79	7		0.3	0.3
	D3S3582, (MYL3)	220–236	FAM	68	7		0.2	0.2
MP3 (30 cycles)	MYO I-CA, (MYH7)	90–102	FAM	66	6		0.2	0.2
MP3 (30 cycles)	MYO II-CA, (MYH7)	108–132	HEX	81	13		0.2	0.2
MP4 (30 cycles)	D1S2622, (TNNT2)	165–189	TET	79	11		0.2	0.2
	TNNT2 Ins/Del, (TNNT2)	335–339	FAM	50	2		0.1	0.1
	D1S2872, (TNNT2)	187–207	FAM	76	11		0.4	0.4
MP5 (30 cycles)	D12S1583, (MYL2)	219–247	HEX	87	14		0.1	0.1
	D15S153, (TPM1)	194–226	TET	86	12		0.1	0.1
	D15S1036, (TPM1)	118–140	TET	85	10		0.2	0.2
MP6 (35 cycles)	D12S1343, (MYL2)	190–216	FAM	70	9		0.2	0.2
	D12S84, (MYL2)	199–219	TET	83	9		0.2	0.2
	HTMα-CA, (TPM1)	108–120	TET	60	6		0.1	0.1
MP7 (35 cycles)	D7S505, (FHC locus)	262–278	FAM	69	8		0.2	0.2
MP7 (35 cycles)	D7S483, (FHC locus)	166–188	HEX	81	10		0.2	0.2
MP8 (35 cycles)	D3S1578, (MYL3)	140–166	FAM	87	14		0.2	0.2
MP8 (35 cycles)	MYBPC3-CA, (MYBPC3)	282–290	HEX	52	5		0.2	0.2
MP9 (35 cycles)	D11S1785, (MYBPC3)	268–276	FAM	70	5		0.15	0.15
	D11S903, (MYBPC3)	99–109	FAM	74	6		0.2	0.2
	D11S1344, (MYBPC3)	273–293	HEX	80	11		0.3	0.3
	D11S4191, (MYBPC3)	111–135	TET	87	11		0.2	0.2
MP10 (35 cycles)	ACTC-CA, (ACTC)	70–96	TET	86	12		0.2	0.2
	D19S887, (TNNI3)	246–262	HEX	72	8		0.3	0.3
	D19S254, (TNNI3)	110–150	TET	60	5		0.4	0.4
	D19S210, (TNNI3)	165–177	TET	73	6		0.2	0.2

Primer sequences of the polymorphic markers were obtained from the Genome Database (GDB)³⁷ except forMYBPC3-CA where primers were designed in accordance with the published genomic sequence (base position 11 473–11 756 (F-5′-GATTTATTTGGCCAGGGAACC-3′; R-5′-TTTCAAAACTGGGGGAACATC-3′)).26