Table 1

PCR amplification of individual exons of the ELOVL4 gene

Exon Forward primers 5′→3′ Reverse primers 5′→3′ bp °C
1 1-150tctctctgggtctccgctt †gaggggaggccttaacattc34758
2 1-150agccacttgcaggagtcagt †tgatggttttacacattctcattttt45264
3 1-150agcaatcggaatgcatgaaa †ttttcacagactggggccta30658
4 1-150tccatgccttgtacattttgtg †tccactgaacacatatatgcaat34860
5 1-150acatctcagtggcttactgcc †gaaaattgtctaaaatgacattgcac29166
6 1-150gaagatgccgatgttgttaaaag †gtcaacaacagttaaggccca37662

  • All the primers had the universal M13 primers attached 5′.

  • ↵1-150 M13F: cgccagggttttcccagtcacgac and †M13R: tttcacacaggaaacagctatgac.