Exon | Forward primers 5′→3′ | Reverse primers 5′→3′ | bp | °C |
1 | 1-150tctctctgggtctccgctt | †gaggggaggccttaacattc | 347 | 58 |
2 | 1-150agccacttgcaggagtcagt | †tgatggttttacacattctcattttt | 452 | 64 |
3 | 1-150agcaatcggaatgcatgaaa | †ttttcacagactggggccta | 306 | 58 |
4 | 1-150tccatgccttgtacattttgtg | †tccactgaacacatatatgcaat | 348 | 60 |
5 | 1-150acatctcagtggcttactgcc | †gaaaattgtctaaaatgacattgcac | 291 | 66 |
6 | 1-150gaagatgccgatgttgttaaaag | †gtcaacaacagttaaggccca | 376 | 62 |
All the primers had the universal M13 primers attached 5′.
↵1-150 M13F: cgccagggttttcccagtcacgac and †M13R: tttcacacaggaaacagctatgac.