Amplification conditions, primers, and sizes of PCR products for HERG coding region. PCR programmes (a-g) are given in Methods. The value following programme (a) indicates the annealing temperature for each PCR amplicon. Primers have been reported previously19 23
| Exon | Size (bp) | Primers |
PCR fragment (bp) |
PCR programme |
Special conditions | |||||
| 1 | 76+ | F:GGGCCACCCGAAGCCTAGT19 | 298 | (c) | 1.0 mmol/l | |||||
| R:CCGTCCCCTCGCCAAAGC19 | MgCl2 | |||||||||
| 2 | 231 | F:GGTCCCGTCACGCGCACTCT23 | 312 | (d) | ||||||
| R:TTGACCCCGCCCCTGGTCGT23 | ||||||||||
| 3 | 165 | F:TGCCCACTGAGTGGGTGC23 | 288 | (c) | ||||||
| R:TGACCTTGGACAGCTCACAG23 | ||||||||||
| 4 | 444 | F:CTCCGGGGCTGCTCGGGAT19 | 284 | (d) | HotStarTaq | |||||
| R:CACCAGCGCACGCCGCTCCT19 | Q solution | |||||||||
| F:CCCTGGACGAAGTGACAGCCAT23 | 318 | (d) | HotStarTaq | |||||||
| R:GGCTGGGGCGGAACGGGTCC23 | Q solution | |||||||||
| 5 | 212 | F:GCCTGACCACGCTGCCTCT23 | 279 | (c) | ||||||
| R:CCTCCAAGGTGAGAGGAGA23 | ||||||||||
| 6 | 429 | F:CAGAGATGTCATCGCTCCTG19 | 295 | (e) | ||||||
| R:CAGGCGTAGCCACACTCGGTAG19 | ||||||||||
| F:TTCCTGCTGAAGGAGACGGAAG19 | 296 | (e) | ||||||||
| R:TACACCACCTGCCTCCTTGCTGA19 | ||||||||||
| 7 | 388 | F:TGCCCCATCAACGGATGTGC23 | 240 | (c) | HotStarTaq | |||||
| R:CAGCCAGCCGATGCGTGAGTCCA23 | ||||||||||
| F:TAGCCTGCATCTGGTACGC23 | 276 | (a) 58°C | HotStarTaq | |||||||
| R:GCCCGCCCCTGGGCACACTCA23 | ||||||||||
| 8 | 200 | F:CTGTGGGTGGGTGGGGTCC23 | 300 | (c) | ||||||
| R:TTTCCCAGCCTGCCACCCACT23 | ||||||||||
| 9 | 253 | F:CCAAGGGAGGGTGTGCTGAG23 | 331 | (c) | ||||||
| R:GGCATTTCCAGTCCAGTGC23 | ||||||||||
| 10 | 194 | F:AGAAGGTGCCTGCTGCCTG23 | 286 | (c) | HotStarTaq | |||||
| R:TCCAGCTCAGGGCAGCCAA23 | ||||||||||
| 11 | 100 | F:AAGGGCCCTGATACTGATTTT23 | 189 | (c) | ||||||
| R:TTCCAGCTCCCAGCCTCA23 | ||||||||||
| 12 | 273 | F:CCCCTCTTTGAGGCCCATT23 | 279 | (a) 55°C | ||||||
| R:CTTCTCGCAGTCCTCCATCA23 | ||||||||||
| F:TGAGAGCAGTGAGGATGA23 | 205 | (a) 58°C | HotStarTaq | |||||||
| R:TAGACGCACCACCGCTGCC23 | Q solution | |||||||||
| 13 | 187 | F:CTCACCCAGCTCTGCTCTCTG19 | 273 | (d) | HotStarTaq | |||||
| R:CACCAGGACCTGGACCAGACT19 | Q solution | |||||||||
| 14 | 178 | F:GTGGAGGCTGTCACTGGTGT19 | 258 | (d) | ||||||
| R:GAGGAAGCAGGGCTGGAGCTT19 | ||||||||||
| 15 | 150+ | F:TGCCCATGCTCTGTGTGTATTG19 | 232 | (e) | ||||||
| R:CGGCCCAGCAGCGCCTTGATC19 | ||||||||||