Table 1

Nucleotide changes in the APC gene detected in tumours and cell lines. A total of 22 unaffected genomic DNA samples, 23 sporadic glioblastomas, 18 sporadic medulloblastomas, five CNS tumour derived cell lines, and one medulloblastoma from an APC patient (see Materials and methods) were examined

Position Source of DNA Base change Amino acid change
Exon 0.1nt 26Unaffected 5Heterozygous C→G
Exon 0.1nt 26Unaffected 10Homozygous C→G
nt 90Heterozygous G→T
Exon 0.1nt 26Unaffected 11Heterozygous C→G
Exon 0.1nt 50Unaffected 14Heterozygous C→T
Exon 0.1nt 26Unaffected 15Homozygous C→G
Exon 0.1nt 26Unaffected 16Homozygous C→G
Exon 0.1nt 26Unaffected 17Heterozygous C→G
Exon 0.1nt 26Unaffected 37Homozygous C→G
nt 17Heterozygous G→A
Exon 0.1nt 26Glioblastoma 93445Heterozygous C→G
Exon 0.1nt 26Medulloblastoma 1835Heterozygous C→G
Exon 0.1nt 26Medulloblastoma 1833Heterozygous C→G
Exon 0.1nt 26SW1088 (astrocytoma)Heterozygous C→G
Exon 0.1nt 34Glioblastoma 93133Heterozygous addition of G
Exon 0.1nt 34Medulloblastoma 1838Heterozygous addition of G
Exon 0.2nt 38Medulloblastoma 1833Heterozygous T→G
Exon 0.2nt 38Medulloblastoma 1837Homozygous T→G
Exon 0.2nt 38SW1088 (astrocytoma)Homozygous T→G
Exon 0.3nt 148SW1088 (astrocytoma)Heterozygous G→ACys→Tyr
Exon 15nt 4326Medulloblastoma 1836Heterozygous T→A
Exon 15nt 3460Medulloblastoma 1840Heterozygous 3 bp deletionLoss of Glu
Exon 15nt 4326Medulloblastoma 1843Heterozygous T→A
Exon 15nt 4326Medulloblastoma 1847Heterozygous T→A
Exon 15nt 3188APC medulloblastomaHeterozygous GTGA deletionStop at nt 3373
nt 3187Heterozygous A deletionStop at nt 3373