DGGE specific intronic PPARγ primers flanking each of the exons
| PPARγ Exon II | 259 bp1-150 | 43.52% UF1-151 |
| II F: (62 nt—40 gc clamp) | ||
| CgCCCgCCgCgCCCCgCgCCCgTCCCgCCgCCCCCgCCCg | ||
| TTTTAACggATTgATCTTTTgC | ||
| II R: (25 nt—5 gc clamp) | ||
| gCgCg | ||
| ACCCTTACATAAATGCCCCC | ||
| PPARγ Exon 1A | 205 bp | 48.00% UF |
| 1A F: (63 nt—40 gc clamp) | ||
| CgCCCgCCgCgCCCCgCgCCCgTCCCgCCgCCCCCgCCCg | ||
| TCTgAAACTCTgTgAgATTgCTg | ||
| 1A R: (20 nt) | ||
| AggAgTgggAgTggTCTTCC | ||
| PPARγ Exon 1B | 283 bp | 35.20% UF |
| 1B F: (20 nt) | ||
| CTCCgTggATCTCTCCgTAA | ||
| 1B R: (72 nt—40 gc, 10 at clamp) | ||
| CgCCCgCCgCgCCCCgCgCCCggCCCgCCgCCCCCgCCCgAAATAATAAA | ||
| CTgAgAgATgAggTCCAATTCT | ||
| PPARγ Exon 2A | 284 bp | 42.24% UF |
| 2A F: (70 nt—40 gc, 10 at clamp) | ||
| CgCCCgCCgCgCCCCgCgCCCggCCCgCCgCCCCCgCCCgAAATAATAAA | ||
| TTgCCCTgTTgCCTTTTTAg | ||
| 2A R: (20 nt) | ||
| gCATCCTTCACAAgCATgAA | ||
| PPARγ Exon 2B | 187 bp | 32.64% UF |
| 2B F: (22 nt—2 gc clamp) | ||
| Cg | ||
| TCATggCAATTgAATgTCgT | ||
| 2B R: (60 nt—40 gc clamp) | ||
| CgCCCgCCgCgCCCCgCgCCCgTCCCgCCgCCCCCgCCCg | ||
| ggggTTCTgCTgAAATgAAA | ||
| PPARγ Exon 3 | 318 bp | 35.84% UF |
| 3 F: (25 nt—5 gc clamp) | ||
| gCgCg | ||
| TAggTTgCTgCTTCCATgTg | ||
| 3 R: (70 nt—40 gc, 10 at clamp) | ||
| CgCCCgCCgCgCCCCgCgCCCggCCCgCCgCCCCCgCCCgAAATAATAAA | ||
| TCCACTggTCTggCAgCTAT | ||
| PPARγ Exon 4A | 241 bp | 54.4% UF |
| 4A F: (70 nt—40 gc, 10 at clamp) | ||
| CgCCCgCCgCgCCCCgCgCCCggCCCgCCgCCCCCgCCCgAAATAATAAA | ||
| gggCTTCTACTgTgTgggAA | ||
| 4A R: (25 nt—5 gc clamp) | ||
| gCgCg | ||
| ACTCTggATTCAgCTggTCg | ||
| PPARγ Exon 4B | 270 bp | 54.08% UF |
| 4B F: (25 nt—5 gc clamp) | ||
| gCgCg | ||
| gAgAAggAgAAgCTgTTggC | ||
| 4B R: (60 nt—40 gc clamp) | ||
| CgCCCgCCgCgCCCCgCgCCCgTCCCgCCgCCCCCgCCCg | ||
| AAACCCAAAACAACTTCCCg | ||
| PPARγ Exon 5A | 222 bp | 40.96% UF |
| 5A F: (69 nt—40 gc, 10 at clamp) | ||
| CgCCCgCCgCgCCCCgCgCCCggCCCgCCgCCCCCgCCCgAAATAATAAA | ||
| ACCTgggATggCATTCACT | ||
| 5A R: (21 nt—2 gc clamp) | ||
| gC | ||
| ATggCCACCTCTTTgCTCT | ||
| PPARγ Exon 5B | 200 bp | 58.56% UF |
| 5B F: (25 nt—5 gc clamp) | ||
| gCgCg | ||
| AgTTCAAACACATCACCCCC | ||
| 5B R: (61 nt—40 gc clamp) | ||
| CgCCCgCCgCgCCCCgCgCCCgTCCCgCCgCCCCCgCCCg | ||
| TTggTCgTTCAAgTCAAgATT | ||
| PPARγ Exon 5C | 263 bp | 45.76% UF |
| 5C F: (60 nt—40 gc clamp) | ||
| CgCCCgCCgCgCCCCgCgCCCgTCCCgCCgCCCCCgCCCg | ||
| TATgCCAAAAgCATTCCTgg | ||
| 5C R: (25 nt—5 gc clamp) | ||
| gCgCg | ||
| gCAAACTCAAACTTgggCTC | ||
| PPARγ Exon 5D | 215 bp | 37.76% UF |
| 5D F: (20 nt) | ||
| CgAAAgCCTTTTggTgACTT | ||
| 5D R: (70 nt—40 gc, 10 at clamp) | ||
| CgCCCgCCgCgCCCCgCgCCCggCCCgCCgCCCCCgCCCgAAATAATAAA | ||
| ACCATCATCCCACCCTCTTT | ||
| PPARγ Exon 6A | 307 bp | 55.68% UF |
| 6A F: (70 nt—40 gc, 10 at clamp) | ||
| CgCCCgCCgCgCCCCgCgCCCggCCCgCCgCCCCCgCCCgAAATAATAAA | ||
| CTgAACCCCCTgTTgTgTTT | ||
| 6A R: (20 nt) | ||
| gAgCgggTgAAgACTCATgT | ||
| PPARγ Exon 6B | 198 bp | 49.60% UF |
| 6B F: (20 nt) | ||
| TgCAggTgATCAAgAAgACg | ||
| 6B R: (71 nt—40 gc, 10 at clamp) | ||
| CgCCCgCCgCgCCCCgCgCCCggCCCgCCgCCCCCgCCCgAAATAATAAA | ||
| ggTgTCAgATTTTCCCTCAgA | ||