Protein truncation test PCR amplification of human CBP cDNA
| Fragment | CBP nucleotides | Primers | Primer sequence |
|---|---|---|---|
| A | -15 - 1155 | pRT7 | 5′-GGATCCTAATACGACTCACTATAGGGCGCGAGCAGGTGAAAATGG-3′ |
| pRT6 | 5′-GCGAGCAGGCCCGAACCTCTC-3′ | ||
| B | 739 - 2125 | pRT38 | 5′-GCTAATACGACTCACTATAGGAACAGACCACCATGGTTTCCCCGCAAATGACTGGTCAC-3′ |
| pRT36 | 5′-ACAGGGGTCCATTTGGAGGTCTC-3′ | ||
| C | 1794 - 2687 | pRT41 | 5′-GCTAATACGACTCACTATAGGAACAGACCACCATGGACCTGCGGAGCCATCTAGTGCAT-3′ |
| pRT40 | 5′-CCGGAAGACGACACAGGAGTTG-3′ | ||
| D | 2469 - 3958 | pRT43 | 5′-GCTAATACGACTCACTATAGGAACAGACCACCATGGTGCCTGGTGCTGCTCTTCCTAAC-3′ |
| PRT32 | 5′-TTCGAGGTCTGCCAGTTTTCTTCAA-3′ | ||
| E1 | 3776 - 4640 | pRT8 | 5′-GGATCCTAATACGACTCACTATAGGGAGACCACCATGGAGACGACAATTTCAAAGGATCAG-3′ |
| pRT2 | 5′-TTGGGCCAGAAATCACCTTCAAA-3′ | ||
| E2 primary | 3284 - 4640 | pRT45 | 5′-GCTAATACGACTCACTATAGGAACAGACCACCATGGTGCCAACCCTAGAAGCACTGTATCG-3′ |
| pRT2 | 5′-TTGGGCCAGAAATCACCTTCAAA-3′ | ||
| E2 booster | 3284 - 4409 | pRT45 | 5′-GCTAATACGACTCACTATAGGAACAGACCACCATGGTGCCAACCCTAGAAGCACTGTATCG-3′ |
| PRT33 | 5′-TGCCCTGTCACATACCCTAATTTC-3′ | ||
| F | 4340 - 5519 | pRT46 | 5′-GCTAATACGACTCACTATAGGAACAGACCACCATGGCAGCCGTTTACCATGAGATCCTTATTG-3′ |
| pRT23 | 5′-ACGGGGCATTTGTTTTCTTGG-3′ | ||
| G primary | 4866 - 5671 | pRT31 | 5′-TGCCACCATGGAGAAGCACAAG-3′ |
| pRT10 | 5′-CGGGCGGTGCTGAGGTAGGAG-3′ | ||
| G booster | 5100 - 5671 | pRT56 | 5′-GCTAATACGACTCACTATAGGAACAGACCACCATGGACCGCTTTGTCTACACCTGCAACG-3′ |
| pRT10 | 5′-CGGGCGGTGCTGAGGTAGGAG-3′ | ||
| H primary | 4599 - 7395 | pRT18 | 5′-CCAAGAAAACAAATGCCCCGT-3′ |
| pRT15 | 5′-GATGCCTGGATTTTCAGTACAAA-3′ | ||
| H booster | 5507 - 7381 | pRT62 | 5′-GCTAATACGACTCACTATAGGAACAGACCACCATGGACAAATGCCCCGTGCCCTTC-3′ |
| pRT63 | 5′-CAGTACAAAAGGTCCAAGAACATGAAAGG-3′ | ||
| Stop | 7327 - 7329 | (TAG) |
First primer of each set has a forward orientation, the second reverse. Positions relative to the CBP cDNA are given, in which position 1 represents the translation start. Nucleotide numbers represent the complete PCR product, including both primers. Endogenous (pRT7) or artificial translation start sites (ATG) are underlined. Start sites are preceded by an eukaryotic T7 promoter sequence and a Kozak sequence.